Mouse stro 1

Cells from control and MFR conditions were lysed using cell lysis buffer (Pierce Protein Research Products, Rockford, IL, Cat. No.: 89900) containing protease inhibitors. Samples were homogenized, and the resulting lysates were centrifuged to obtain clear supernatant. Protein concentration was determined by BCA protein assay (Pierce BCA Protein Assay Kit, Rockford, IL, prod # 23225). Fifty microgram of protein lysate from each sample was resolved by SDS-PAGE gel electrophoresis and data analyzed, following our previously described methods ^{11 (link), 19 (link)}. The specific primary antibodies used included PPARγ (H-100, Cat. No.: sc-7196, 1:200), adipocyte differentiation-related protein (ADRP) (H-80, (Cat. No.: sc-32888, 1:150), CCAAT/enhancer binding protein α (C/EBPα) (14AA, Cat. No.: sc-61, 1:200), β-catenin (E-5, Cat. No.: sc-7963, 1:250), lymphoid enhancer-binding factor 1 (LEF1) (H-70, Cat. No.: sc-28687, 1:250), All of the above primary antibodies were purchased from Santa Cruz Biotechnology, Inc, Santa Cruz, CA. Mouse stro-1 (1:500) (eBioscience, Cat. No.: 14-6688-80) and goat anti-Rat endoglin/CD105 antigen affinity-purified polyclonal antibody (Novus, Cat. No.: AF6440) (1:1000) were purchased from their respective vendors.