Bronchoalveolar lavage fluid (BALF) was obtained by tracheal intubation, injection and recovery of sterile PBS. BALF was centrifuged for 10 min at 4°C for 2000 rpm. The total number of cells was measured by Hematology analyzer (Abbott CELL-DYN 4000). BALF cell smears were stained with Giemsa, and the neutrophils and macrophages were counted.
Cell dyn 4000
Manufactured by Abbott
Sourced in United States
The CELL-DYN 4000 is a hematology analyzer developed by Abbott. It is designed to perform complete blood count (CBC) analysis and differential cell count. The CELL-DYN 4000 utilizes advanced technology to provide accurate and reliable results for clinical laboratory testing.
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9 protocols using cell dyn 4000
1
Quantifying Airway Inflammatory Cells
2
Measurement of Blood Biomarkers: Protocols
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The measurement of blood biomarkers is described in detail elsewhere43 (link). WBC was performed on freshly collected blood samples. Plasma aliquots were immediately stored at −70 ℃ until being tested and blood samples were centrifuged for 15 min at 4 ℃. Three biomarkers were considered in this study: Interleukin-6 (IL-6), white blood cells (WBC), and tumor necrosis factor-soluble receptor-II (sTNF-RII). IL-6 and sTNF-RII were analyzed with enzyme-linked immunosorbent assay (Quantikine, R&D Systems) at Immunology, Asthma, and Allergy Research Institute, Tehran University of Medical Sciences (Tehran, Iran). WBC of whole blood was counted using an automatic hematological analyzer (CellDyn 4000, Abbott). All samples were analyzed in duplicate to ensure reproducibility. The blood sampling tube was made of plastic. Centrifuges are used for biomarkers (The g force of the centrifuge was 1100). No centrifuge was used for white blood cells. In this study, both EDTA and citrate tubes were used. EDTA tubes were used to measure white blood cells and citrate tubes were used to measure blood biomarkers.
3
Cardiovascular Risk Biomarkers in ER
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BPs was measured using the first and fifth phases of the Korotkoff sound, by a cuff sphygmomanometer. Body mass index (BMI) was calculated as the weight divided by the height squared (kg/m2). The body surface area (BSA, m2) of each patient and other clinical characteristics were also recorded. Blood samples were drawn from the antecubital vein as soon as patients were admitted to ED. All of the measurements were performed within 1 h of venipuncture to prevent in-vitro platelet activation. Blood specimens were collected into ethylenediaminetetraacetic acid (EDTA) containing tubes and analyzed with the aid of a Cell-Dyn 4000 cell counter (Abbott; North Chicago, IL, USA) using commercial kits (Abbott, USA). The normal MPV was accepted as 6.8–10.8 fL. NLR was calculated from the levels of neutrophils and lymphocytes, manually. The levels of plasma triglycerides, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, creatinine, glucose, hemoglobin, and the creatine-kinase MB isoenzyme were measured using commercial kits. Hs-CRP levels were measured with the aid of an Aeroset autoanalyzer using a commercial spectrophotometric kit (Abbott, USA).
4
Exhaled Nitric Oxide and Blood Cell Counts
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Exhaled nitric oxide at an exhalation flow of 50 mL/s (FENO50) was measured with NIOX VERO (Circassia AB, Solna, Sweden), NIOX Flex (Aerocrine AB, Solna, Sweden) or Eco Medics CLD 88 (Eco Medics, Duernten, Switzerland). The measurements were performed in accordance with current recommendations.12 (link)
Blood neutrophil count (B-Neu) and blood eosinophil count (B-Eos) were analysed (Cell-Dyn 4000; Abbott Laboratories, Abbott Park, IL, USA and Sysmex XN-10; Sysmex America, Inc., Lincolnshire, IL, USA).
Blood neutrophil count (B-Neu) and blood eosinophil count (B-Eos) were analysed (Cell-Dyn 4000; Abbott Laboratories, Abbott Park, IL, USA and Sysmex XN-10; Sysmex America, Inc., Lincolnshire, IL, USA).
5
Investigating WBC Subsets and Cortisol Levels
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After an overnight fast, blood samples were collected in heparinized tubes between 8:00 a.m. and 9:00 a.m. and immediately transported to the laboratory. To determinate the WBC subset counts, the total number of WBC and leukocyte differential counts were calculated with a Coulter counter (CELL-DYN 4000; ABBOTT). Enumeration by flow cytometry with FACS count (Becton Dickinson) included the following cells: two types of T cell subsets CD4, CD8.
Serum levels of cortisol were measured using a competitive enzyme immunoassay which was performed entirely within the ST AIA-PACK CORT (Tosoh Bioscience).
Serum levels of cortisol were measured using a competitive enzyme immunoassay which was performed entirely within the ST AIA-PACK CORT (Tosoh Bioscience).
6
Comprehensive Blood Panel Analysis
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Blood samples were collected after a minimum fasting period of 8 hours. White blood cells; platelet count; levels of hematocrit, aspartate transaminase, alanine transaminase, serum creatinine, alkaline phosphatase, and serum iron; TIBC; and hemoglobin level were measured using CellDyn 4000 (Abbott Diagnostics, Abbott Park, IL, USA). Serum iron level and TIBC were measured using the FerroZine method [18 (link)]. All laboratory examinations were conducted at the same center.
7
Analytical Biomarkers in Blood Samples
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Blood samples were collected at the baseline visit and were obtained at 8:00 a.m. after an overnight fast and abstinence from smoking. Blood counts were analyzed immediately in an automated cell counter (Cell-Dyn 4000; Abbott Diagnostics, Abbott Park, IL, USA), at Sahlgrenska University Hospital, Gothenburg, Sweden. Serum and plasma samples were frozen within 1 h, and stored at − 80 °C until required for analysis. Methods regarding concentrations of EPO, iFGF23, serotonin, osteocalcin, N-terminal propeptide of type I collagen (P1NP), iron, ferritin, total iron-binding capacity (TIBC), 25(OH)D, ALP, C-reactive protein (CRP), intact parathyroid hormone (iPTH), estradiol, estimated glomerular filtration rate (eGFR), and calculation using a cystatin C-based formula have previously been described [18 (link)–25 (link)]. Iron deficiency was defined as ferritin < 20 μg/L or transferrin saturation < 15%. As 25(OH)D varies according to season, a z-score was calculated and an expected value of 25(OH)D was attained for each participant according to season [26 (link)] and was used in statistical analysis of 25(OH)D values.
8
Baseline Biomarker Assessment in Fasted Subjects
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Blood samples were collected at the baseline visit, at around 8.00 a.m., following an overnight fast and abstinence from smoking. Hb was analyzed immediately in an automated cell counter (CellDyn 4000; Abbott Diagnostics, Abbott Park, IL, USA), at Sahlgrenska University Hospital, Gothenburg, Sweden. Plasma and serum samples were frozen within 1 h and stored at − 80 °C until required for analysis. Methods regarding plasma/serum concentrations of intact fibroblast growth factor 23 (iFGF23), glucose, osteocalcin, N-terminal propeptide of type 1 collagen (P1NP), testosterone, estradiol, erythropoietin (EPO), C-reactive protein (CRP), intact parathyroid hormone (iPTH), 25(OH)D, and cystatin C, as well as calculation using cystatin C-based formula for estimated glomerular filtration rate (eGFR), have previously been described [20 (link)–23 (link)]. The serum levels of alkaline phosphatase (ALP), albumin-adjusted calcium, and phosphate were analyzed according to the routine laboratory technique used at Sahlgrenska University Hospital. Hypogonadism was defined as testosterone levels < 8 nmol/L.
9
Biomarker Measurements in Large Cohort Study
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