Insc gal4

Manufactured by BD

The Insc-Gal4 is a lab equipment product designed for genetic manipulation in research applications. It serves as a tool for targeted gene expression in model organisms. The core function of the Insc-Gal4 is to facilitate the controlled activation of genes of interest.

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Lab products found in correlation

Elav gal4, by BD (1 mentions) Rtf1 rnai, by BD (1 mentions) Rtf1 rnai, by Vienna Drosophila Resource Center (1 mentions) Actin5c gal4, by BD (1 mentions) Tub gal4, by BD (1 mentions) Uas dcr2, by BD (1 mentions) Repo gal4, by BD (1 mentions)

4 protocols using insc gal4

Drosophila Genetic Crosses and Fly Stocks

Cited 3 times

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Fly stocks and genetic crosses were reared at 25°C unless otherwise stated. Fly stocks were kept in vials or bottles containing standard fly food (0.8% Drosophila agar, 5.8% Cornmeal, 5.1% Dextrose, and 2.4% Brewer’s yeast). The following fly strains were used in this study: insc-Gal4 (BDSC#8751; 1407-Gal4), insc-Gal4, UAS-Dicer2 with and without UAS-CD8-GFP, Jupiter-GFP (G147), UAS-hyx, UAS-HRPT2 [51 (link)]. The type II NSC driver: w; UAS-Dicer 2, wor-Gal4, ase-Gal80/CyO; UAS-mCD8-GFP/TM3, Ser [86 (link)]; hyx RNAi hyx^HT622, UAS-venus-polo [28 (link)].
The following stocks were obtained from Bloomington Drosophila Stock Center (BDSC): UAS-Gal RNAi (BDSC#50680; this stock is often used as a control UAS element to balance the total number of UAS elements), ctr9^12P023 (BDSC#59389), rtf1 RNAi (BDSC#36586), rtf1 RNAi (BDSC#34850) [87 (link)], rtf1 RNAi (BDSC#31718), UAS-aurA (BDSC#8376), actin5C-Gal4 (BDSC#25374).
The following stocks were obtained from Vienna Drosophila Resource Center (VDRC): hyx RNAi (28318), hyx RNAi (103555), atms RNAi (108826) [87 (link)], atu RNAi (17490) [88 (link)], atu RNAi (106074), ctr9 RNAi (108874), ctr9 RNAi [89 (link)], rtf1 RNAi (27341) [88 (link)], rtf1 RNAi (110392).
All experiments were carried out at 25°C, except for RNAi knockdown or overexpression experiments that were performed at 29°C.

Deng Q., Wang C., Koe C.T., Heinen J.P., Tan Y.S., Li S., Gonzalez C., Sung W.K, & Wang H. (2022). Parafibromin governs cell polarity and centrosome assembly in Drosophila neural stem cells. PLoS Biology, 20(10), e3001834.

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Drosophila Genetic Manipulation Protocols

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Fly stocks and genetic crosses were raised at 25°C unless otherwise stated. Fly stocks were kept in vials or bottles containing standard fly food (0.8% Drosophila agar, 5.8% Cornmeal, 5.1% Dextrose and 2.4% Brewer’s yeast). The following fly strains were used: vib¹³³, vib¹¹⁰⁵, UAS-NYFP-Myc, UAS-CYFP-HA, UAS-NYFP-Myc-Vib, UAS-CYFP-HA-Sqh, UAS-Vib::Venus, UAS-VibT63A::Venus, UAS-VibT63E::Venus, UAS-PITPα::Venus and UAS-PITPβ::Venus (this study), fwd³, P{w⁺, PI4KIIIα} FRT80B and PI4KIIΔ (Julie, A. Brill), sqh^Ax3; p[w;sqh-GFP42], UAS-Cdc42::GFP (Akira Chiba), baz, FRT19A, w, Ubi-Rok-GFP (I) and w;; Ubi-Rok-GFP (III) (Bellaiche, Y), UAS-WAVE-HA-CYFP and UAS-Abi-Myc-NYFP(Bogdan, S). The following stocks were obtained from Bloomington Drosophila Stock Center: insc-Gal4 (BDSC#8751), elav-Gal4 (BDSC#8765), P{lacW}vib^j5A6/TM6B,Tb (BDSC#12144), P{lacW}vib^j7A3/TM3, Sb (BDSC#10308), Vib deficiency w; Df(3R)BSC850/TM6C,Sb,cu (BDSC#27922), sqh^AX3,FRT19A/FM7c (BDSC#25712), sqh RNAi (BDSC#33892), w; Sqh-mCherry (BDSC#59024), PI4KIIIα^C, FRT19A/FM7c (BDSC#57112), w; UAS-2xOsh2PH-GFP (BDSC#57353), w; UAS-2xOsh2PH-GFP/CyO; Pri/TM6B, Tb (BSDC#57352).
Crosses for RNAi knockdown and overexpression were incubated at 25°C for 24 hr before transferring to 29°C, where the larvae were aged for 72 hr. Wandering third instar larvae were dissected and processed for immunohistochemistry staining.

Koe C.T., Tan Y.S., Lönnfors M., Hur S.K., Low C.S., Zhang Y., Kanchanawong P., Bankaitis V.A, & Wang H. (2018). Vibrator and PI4KIIIα govern neuroblast polarity by anchoring non-muscle myosin II. eLife, 7, e33555.

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Drosophila Germline Stem Cell RNAi

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UAS-RNAi lines are generated by TRiP and are available at the Bloomington Drosophila stock center (BDSC). For the RNAi experiments we used a maternal triple driver MTD-Gal4 (BDSC 31777) or UAS-dcr2; nanos-Gal4 (BDSC 25751) to drive expression of UAS-RNAi transgenes in GSCs, MAT-Gal4 (BDSC 7063) for germline expression outside the germarium, and insc-Gal4 for expression in larval Nbs. For trap-mediated loss of function analyses we used UAS-shRNAs targeting eGFP as previously described (Neumuller et al., 2012 (link)). Protein trap lines scny::GFP, dom::GFP and CG11266::GFP are described in (Buszczak et al., 2007 (link)). We chose the name inselgruppe (German for ‘group of islands’, abbreviated: igru) due to the few remaining, scattered Vasa positive cells observed in the ovaries upon knockdown.

Yan D., Neumüller R.A., Buckner M., Ayers K., Li H., Hu Y., Yang-Zhou D., Pan L., Wang X., Kelley C., Vinayagam A., Binari R., Randklev S., Perkins L.A., Xie T., Cooley L, & Perrimon N. (2014). A regulatory network of Drosophila germline stem cell self-renewal. Developmental cell, 28(4), 459-473.

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Investigating Drosophila DNA Damage Repair

Cited 1 time

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The following fly stocks were used in this study: UAS-DDB1, UAS-DDB1^RNAi-Res, ddb1^HK-2-3, ddb1^W197 (generated in this study), ddb1^5-1, cul4^G1-3, cul4^JJ11, UAS-Flag-Cul4, UAS-Flag-Cul4^KR (CT Chien); mahj¹ [36 (link)]; UAS-Myc-Mahj, UAS-Myc-Mahj^R1120/1123E (this study). The following fly strains were obtained from BDSC: β-gal^RNAi (#50680), UAS-CD8-GFP (#32186), wts^RNAi (#34064), mahj^RNAi1 (#34912) and UAS-Yki^S168A (#28818); RNAi lines including ddb1^RNAi (#44974) and cul4^RNAi1 (#105668), cul4^RNAi2 (#44829) and mahj^RNAi2 (#110669) were obtained from the VDRC. Df(2R) XE2900 (#108418) is from the Kyoto Drosophila Genomics and Genetic Resource.
NSC drivers included insc-Gal4 (BDSC#8751; 1407-Gal4) or insc-Gal4, tub-Gal80^ts. Glial driver was repo-Gal4 (BDSC# 7145). Ubiquitous driver was tub-Gal4 (BDSC#5138). UAS-Dcr2 (BDSC#24650) was used together with various RNAi stocks. ddb1 and mahj RNAi knockdown efficiency was verified by immunostaining of anti-DDB1 and anti-Mahj antibodies in larval brains.
All experiments with mutants were carried out at 25 °C, and experiments for RNAi knockdown or overexpression were performed at 29 °C.

Ly P.T., Tan Y.S., Koe C.T., Zhang Y., Xie G., Endow S., Deng W.M., Yu F, & Wang H. (2019). CRL4Mahj E3 ubiquitin ligase promotes neural stem cell reactivation. PLoS Biology, 17(6), e3000276.

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