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Parp1 c 2 10

Manufactured by Enzo Life Sciences
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PARP1 (C-2-10) is a recombinant protein product from Enzo Life Sciences. It represents the C-terminal domain of the human PARP1 enzyme. PARP1 is a nuclear enzyme involved in various cellular processes, including DNA repair and programmed cell death.

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Lab products found in correlation

β actin, by Novus Biologicals (2 mentions) Cleaved caspase 3, by Cell Signaling Technology (2 mentions) P21 waf1 cip1, by Cell Signaling Technology (1 mentions) Phospho chk2, by Cell Signaling Technology (1 mentions) Phospho chk1, by Cell Signaling Technology (1 mentions) Ampkα1, by Cell Signaling Technology (1 mentions) P70 s6 kinase, by Cell Signaling Technology (1 mentions) Phospho p70s6, by Cell Signaling Technology (1 mentions) H2a x d17a3 xp, by Cell Signaling Technology (1 mentions) Alexa fluor 488 anti rabbit, by Thermo Fisher Scientific (1 mentions) Phospho 4ebp1, by Cell Signaling Technology (1 mentions) 4e bp1, by Cell Signaling Technology (1 mentions) Phospho ampk, by Cell Signaling Technology (1 mentions) γh2ax ser139, by Cell Signaling Technology (1 mentions) Phospho mtor ser2448, by Cell Signaling Technology (1 mentions) Q vd oph, by Merck Group (1 mentions) Lamp1, by Cell Signaling Technology (1 mentions) N acetyl l cysteine nac, by Merck Group (1 mentions) Erastin, by Cayman Chemical (1 mentions) Llome, by Cayman Chemical (1 mentions)

2 protocols using parp1 c 2 10

1

Comprehensive Molecular Profiling Protocol

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Antibodies against the following proteins were used: phospho-ATM (Ser 1981) (#5883), ataxiatelangiectasia mutated, ATM (#2873), phospho-AMPK (Thr172) (#2535), AMPKα1 (#2795), phospho-AMPK substrates (#5759), ATG7 (#8558), cleaved caspase 3 (#9664), phospho-CHK1 (Ser 345, 133D3) (#2348), CHK1 (2G1D5) (#2360), phospho-CHK2 (Thr 68) (#2197), CHK2 (#2662), p21 Waf1/CIP1 (12D1) (#2947), p27 Kip 1 (D69C12) XP (#3686), phospho 4EBP1 (Thr 37/46) (#9459), 4EBP1 (#9452), phospho-p70S6 kinase (Thr389) (#9205), p70S6 kinase (#9202), γH2AX (Ser139) (#9718), H2A.X (D17A3) XP (#7631) and phospho-MTOR (Ser2448) (#2971) all from Cell Signaling Technology); Ki67 (30-9) (#790-4286) from Roche; PARP1 (C-2-10) (#BML-SA249-0050) from Enzo Life Sciences; p62 (#610832) from BD Biosciences; ATG5 (#0262-100/ATG5-7C6) from Nanotools; ACTIN β (#NB600-501) from Novus Biologicals; LC3 (#M152-3) from MBL; phospho-p62 (Ser403) (#MABC186) from Merck Millipore; p16 (#805-4713) from Ventana; horseradish peroxidase-conjugated anti-rabbit (#111-035-003) and horseradish peroxidase (HRP)-conjugated anti-mouse (#115-035-174) from Jackson ImmunoResearch; and anti-mouse (#A11001) and anti-rabbit Alexa Fluor 488 (#A11008) from Invitrogen.
Beauvarlet J., Bensadoun P., Darbo E., Labrunie G., Rousseau B., Richard E., Draskovic I., Londono-Vallejo A., Dupuy J.W., Nath Das R., Guédin A., Robert G., Orange F., Croce S., Valesco V., Soubeyran P., Ryan K.M., Mergny J.L, & Djavaheri-Mergny M. (2019). Modulation of the ATM/autophagy pathway by a G-quadruplex ligand tips the balance between senescence and apoptosis in cancer cells. Nucleic Acids Research, 47(6), 2739-2756.
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2

Antibody-based analysis of autophagy and apoptosis

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Antibodies against the following proteins were used: ATG5 (#0262-100/ATG5-7C6) from Nanotools (Teningen, Baden-Württemberg, DE), ATG7 (#8558) ), Cleaved-Caspase3 (#9664), LAMP-1 (#9091) from Cell Signaling Technology (Danvers, MA, USA), PARP-1 (C-2-10) (#BML-SA249-0050) from Enzo Life Sciences (Farmingdale, NY, USA), Actin β (#NB600-501) from Novus Biologicals (Centennial, CO, USA), HRP conjugated rabbit (#111-035-003) and HRP conjugated mouse (#115-035-174) from Jackson ImmunoResearch (Ely, Cambridgeshire, UK), Alexa488 conjugated anti-rabbit (#A11008) from Invitrogen (Waltham, MA, USA). Hoechst 33258 (#14530), E64d (#E8640), Propidium iodide (#P4864), Chloroquine diphosphate salt (#C6628), QV-D-Oph (#SML0063), butylhydroxyanisole (BHA, #B1212000), N-acetyl-L-cysteine (NAC, #A8199) were purchased from Sigma-Aldrich (St. Louis, MO, USA).). Tetramethylrhodamine methyl-ester, TMRM (T-668) was purchased from Molecular Probes (Eugene, OR, USA). Fluoromount G (#00-4958-02) was purchased from Invitrogen. 20A was synthesized as previously described [42 (link),43 (link)]. Ferrostatin-1 (#341494) was purchased from Calbiochem (San Diego, CA, USA)), while LLOME (#16008) and Erastin (#17754) were purchased from Cayman Chemical (Ann Arbor, MI, USA).
Beauvarlet J., Nath Das R., Alvarez-Valadez K., Martins I., Muller A., Darbo E., Richard E., Soubeyran P., Kroemer G., Guillon J., Mergny J.L, & Djavaheri-Mergny M. (2020). Triarylpyridine Compounds and Chloroquine Act in Concert to Trigger Lysosomal Membrane Permeabilization and Cell Death in Cancer Cells. Cancers, 12(6), 1621.
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