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Tubocurarine

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Tubocurarine is a neuromuscular blocking agent used in laboratory settings. It acts by competitively inhibiting the binding of acetylcholine to nicotinic acetylcholine receptors at the neuromuscular junction, resulting in muscle relaxation.

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Lab products found in correlation

Pitstop 2, by Abcam (1 mentions) Dynole 34 2, by Abcam (1 mentions) Cytation 5 reader, by Agilent Technologies (1 mentions)

Spelling variants of this product

D-tubocurarine (3 citations) Tubocurarine chloride (2 citations)

2 protocols using tubocurarine

1

Neuregulin-3 Immunostaining Protocol

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BACE-IV was from Calbiochem; Alexa 555-conjugated bungarotoxin was from Thermo Fisher Scientific; tubocurarine was from Tocris; Dynole-34-2 and Pitstop 2 were from Abcam. Goat polyclonal anti-NRG3 against the EGF-L domain was from Neuromics; rabbit polyclonal anti-NRG3 against the NRG3 C-terminus was described previously (Vullhorst et al., 2017 (link)). See also Table S1 for a complete list of primary antibodies used in this study. Secondary antibodies conjugated to fluorophores for immunofluorescence microscopy or to HRP for chemiluminescence-based Western blotting were from Thermo Fisher Scientific or Jackson Immunoresearch.
Ahmad T., Vullhorst D., Chaudhuri R., Guardia C.M., Chaudhary N., Karavanova I., Bonifacino J.S, & Buonanno A. (2022). Transcytosis and trans-synaptic retention by postsynaptic ErbB4 underlie axonal accumulation of NRG3. The Journal of Cell Biology, 221(7), e202110167.
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2

Organoid Contraction Imaging

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Organoids at 8–9 weeks were imaged using phase contrast in a Cytation 5 reader (Biotek) with temperature and CO2 control (37 °C, 5% CO2). Media was changed 1 h prior to the experiment and a single site per well was used to establish a baseline of contractions immediately prior to compound addition. Botulinum neurotoxin type A (6.7 nM final concentration) (List Biological Laboratories, 130 A) and Tubocurarine (12.5 μM) (Tocris, 2820) were resuspended in water, per manufacturer’s instructions.
Pereira J.D., DuBreuil D.M., Devlin A.C., Held A., Sapir Y., Berezovski E., Hawrot J., Dorfman K., Chander V, & Wainger B.J. (2021). Human sensorimotor organoids derived from healthy and amyotrophic lateral sclerosis stem cells form neuromuscular junctions. Nature Communications, 12, 4744.
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