Modified eagle s medium mem

Chemicals utilized for molecular and cellular biology and buffer preparation were purchased from Sigma-Aldrich (St. Louis, MO, USA). Cell culture medium including Dulbecco’s Modified Eagle’s Medium (DMEM; Cat#: 10-013-CVR, Corning, New York, NY, USA), modified Eagle’s Medium (MEM; Cat#: 10-010-CVR, Corning), and supplements including penicillin and streptomycin (Cat#: 15140-122, Gibco, Waltham, MA, USA) were purchased from Life Science Technologies (Rockville, MD, USA). Antibodies for phospho-FGFR3 (Cat#: SC-33041), FGFR3 (Cat#: SC-13121), p-RSK2 T577 (Cat#: SC-16407, SC-377501), RSK2 (Cat#: SC-9986), and β-actin (Cat#: SC-69879) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies for phospho-RSK T359/S363 (Cat#: 9344), phospho-ERK1/2 (Cat#: 9106), ERK1/2 (Cat#: 9102), and Ki-67 (Cat#: 9027) were purchased from Cell Signaling Technology (Beverly, MA, USA) for the western blot analysis, immunohistochemistry, and immunocytofluorescence. Fetal bovine serum (Cat#: 26140-079, Gibco) was purchased from Life Science Technologies and heat inactivated before utilization. Human recombinant bFGF (Cat#: SRP4037, Sigma-Aldrich), dimethylsulfoxide (DMSO, Cat#: D8418, Sigma-Aldrich), and kaempferol (Cat#: ALX-385-005, Enzo, Farmingdale, NY, USA) were purchased from Sigma-Aldrich.

HepG2 cells were purchased from ATCC (Manassas, VA, USA). Modified Eagle’s medium (MEM) was bought from CORNING (10-009CVR). Insulin were from Sigma-Aldrich (I5500-10 mg). C reactive protein (CRP) was bought from APPLYGEN (Beijing, China, C2308). Oleic acid (OA) was purchased from Sigma-Aldrich, O1383.