Panel 1C was acquired with a ZEISS LSM 880 with Airyscan system equipped with a Plan-Apochromat 63×/1.40 Oil DIC M27 objective and using 488 and 561 nm wavelength lasers. Panels 2A,B S3A, S6A and S7B were acquired with a DeltaVision PersonalDV (Applied Precision) with either a Plan Apo 60x/1.42 NA or a UPlanSApo 100x/1.4 NA oil lenses. Panels 2F, 3B,H, S3B,C, S5 and S7A were acquired on a Leica TCS SP5 confocal microscope equipped with a HCX PL APO CS 63.0x/1.40 NA oil lens. FRET experiments were performed on HeLa cells using the Leica FRET AB wizard. Ku70(AF488)/Ku80(488 DyLight)/DNA-PKcs(488 DyLight) served as donors and LMNA (AF594 for Ku80 and DNA-PKcs FRET; AF555 for Ku70 FRET) as acceptor. Panels 3C,F were acquired with a OMX Structured Illumination Super-resolution Scope equipped with a PlanApo 60x/1.40 Oil DIC objective and using 488 and 568 nm wavelength lasers.
Hcx pl apo cs 63.0x 1 40 na oil lens
Manufactured by Leica
The HCX PL APO CS 63.0x/1.40 NA oil lens is a high-performance microscope objective lens manufactured by Leica. It features an apochromatic correction and a numerical aperture of 1.40, which enables the lens to gather a large amount of light and produce high-resolution images. The lens is designed for use with oil immersion, which further enhances its optical performance.
Automatically generated - may contain errors
Lab products found in correlation
Deltavision personaldv, by Cytiva (2 mentions)
Tcs sp5 confocal microscope, by Leica (2 mentions)
Lsm 880 with airyscan system, by Zeiss (2 mentions)
Plan apo 60x 1.42 na, by Cytiva (2 mentions)
Uplansapo 100x 1.4 na, by Cytiva (2 mentions)
Fret ab wizard, by Leica (2 mentions)
Plan apochromat 63 1.40 oil dic m27 objective, by Zeiss (2 mentions)
2 protocols using hcx pl apo cs 63.0x 1 40 na oil lens
1
Multimodal Microscopy Techniques for DNA Repair
2
Multimodal Microscopy Techniques for DNA Repair
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Panel 1C was acquired with a ZEISS LSM 880 with Airyscan system equipped with a Plan-Apochromat 63×/1.40 Oil DIC M27 objective and using 488 and 561 nm wavelength lasers. Panels 2A,B S3A, S6A and S7B were acquired with a DeltaVision PersonalDV (Applied Precision) with either a Plan Apo 60x/1.42 NA or a UPlanSApo 100x/1.4 NA oil lenses. Panels 2F, 3B,H, S3B,C, S5 and S7A were acquired on a Leica TCS SP5 confocal microscope equipped with a HCX PL APO CS 63.0x/1.40 NA oil lens. FRET experiments were performed on HeLa cells using the Leica FRET AB wizard. Ku70(AF488)/Ku80(488 DyLight)/DNA-PKcs(488 DyLight) served as donors and LMNA (AF594 for Ku80 and DNA-PKcs FRET; AF555 for Ku70 FRET) as acceptor. Panels 3C,F were acquired with a OMX Structured Illumination Super-resolution Scope equipped with a PlanApo 60x/1.40 Oil DIC objective and using 488 and 568 nm wavelength lasers.
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