Mice were overdosed on isoflurane gas and perfused with room temperature 0.1M phosphate-buffered solution (PBS), pH 7.3, and then with ice-cold 4% paraformaldehyde (PFA) solution in PBS, 10 days after viral injection surgery. After extraction, the brains were post-fixed in 4% PFA solution overnight. 50 μm thickness slices were obtained using the Integraslice 7550 MM vibrating microtome (Campden Instruments, Loughborough, England), and were stored at 4°C in 0.1M PBS. The slices were mounted onto 25 × 75 × 1 mm frosted microscope slides (Thermo-Scientific, Waltham, MA, United States) using 125 uL ProLong Gold antifade reagent (Invitrogen) as the mountant. The slides were imaged using a Nikon fluorescence microscope (Nikon, Minato, Tokyo, Japan) with images obtained using both 4X and 10X magnification objectives.
125 ul prolong gold antifade reagent
Manufactured by Thermo Fisher Scientific
Sourced in United States
The 125 uL ProLong Gold antifade reagent is a fluorescent mounting medium designed to preserve fluorescent signals in microscopy samples. It helps to prevent photobleaching and maintain the integrity of fluorescent labels.
Automatically generated - may contain errors
2 protocols using 125 ul prolong gold antifade reagent
1
Brain Tissue Preparation and Imaging
2
Brain Tissue Preparation and Imaging
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Mice were overdosed on isoflurane gas and perfused with room temperature 0.1M phosphate-buffered solution (PBS), pH 7.3, and then with ice-cold 4% paraformaldehyde (PFA) solution in PBS, 10 days after viral injection surgery. After extraction, the brains were post-fixed in 4% PFA solution overnight. 50 μm thickness slices were obtained using the Integraslice 7550 MM vibrating microtome (Campden Instruments, Loughborough, England), and were stored at 4°C in 0.1M PBS. The slices were mounted onto 25 × 75 × 1 mm frosted microscope slides (Thermo-Scientific, Waltham, MA, United States) using 125 uL ProLong Gold antifade reagent (Invitrogen) as the mountant. The slides were imaged using a Nikon fluorescence microscope (Nikon, Minato, Tokyo, Japan) with images obtained using both 4X and 10X magnification objectives.
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