Msd 96 well multi array human sclerostin assay

Serum sclerostin levels were measured in blood samples drawn after an overnight fast both at baseline and during hyperinsulinaemia as established during the last hour of the HEC procedure. Sclerostin was measured by the endocrine laboratory of Amsterdam UMC using an electrochemiluminescence assay (MSD 96-well MULTI-ARRAY Human Sclerostin Assay; Meso Scale Discovery), with an intra-assay CV ≤ 6.0% and an interassay CV of 11%. The lower limit of quantitation was 0.040 ng/mL.

Plasma sclerostin was measured with an electrochemiluminescence assay (MSD 96-Well MULTI-ARRAY Human Sclerostin Assay; Meso Scale Diagnostics, Rockville, MD, USA), as described previously [20 (link)]. Plasma glucose, total cholesterol and triglycerides were measured with an automated spectrophotometer (ABX Pentra 400 autoanalyzer; HORIBA, Kyoto, Japan) with enzymatic colorimetric kits. Plasma insulin was determined with commercially available radioimmunoassay kits (human insulin-specific radioimmunoassay, MilliporeSigma, Burlington, MA, USA). Plasma glycosylated hemoglobin (HbA1c) was measured with ion exchange chromatography (Tosoh G8 HPLC analyser; Sysmex, Kobe, Japan).