Log In Sign up
The largest database of trusted experimental protocols

Goat anti rat igg secondary antibody

Manufactured by Southern Biotech
Visit Supplier

Goat-anti-rat IgG secondary antibody is a laboratory reagent used in various immunological techniques. It is a purified antibody generated in goats that binds to the immunoglobulin G (IgG) molecules of rats. This secondary antibody can be used to detect and amplify signals from primary antibodies directed against rat antigens in applications such as Western blotting, ELISA, and immunohistochemistry.

Automatically generated - may contain errors

Lab products found in correlation

Aec substrate solution, by Vector Laboratories (2 mentions)

2 protocols using goat anti rat igg secondary antibody

1

Histological Analysis of Imiquimod-Induced Skin Inflammation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Skin sections from IMQ-treated skin were embedded and frozen in OCT and stained with H&E and Gr-1 mAb for IHC. Epidermal thickness was determined by measuring the average interfollicular distance under the microscope in a blinded manner. For IHC staining, skin cryosections were fixed, blocked and then stained with purified rat-anti-mouse Gr-1 Ab (1:50 dilution) following with goat-anti-rat IgG secondary antibody (1:200 dilution, Southern Biotech). Slides were developed with AEC substrate solution (Vector Laboratories) and then counterstained with hematoxylin. Images were acquired at x200 magnification using Aperio ScanScope digital scanners.
Cai Y., Xue F., Qin H., Chen X., Liu N., Fleming C., Hu X., Zhang H.G., Chen F., Zheng J, & Yan J. (2019). Differential Roles of the mTOR-STAT3 Signaling in Dermal γδ T Cell Effector Function in Skin Inflammation. Cell reports, 27(10), 3034-3048.e5.
+ Open protocol
+ Expand
2

Histological Analysis of Imiquimod-Induced Skin Inflammation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Skin sections from IMQ-treated skin were embedded and frozen in OCT and stained with H&E and Gr-1 mAb for IHC. Epidermal thickness was determined by measuring the average interfollicular distance under the microscope in a blinded manner. For IHC staining, skin cryosections were fixed, blocked and then stained with purified rat-anti-mouse Gr-1 Ab (1:50 dilution) following with goat-anti-rat IgG secondary antibody (1:200 dilution, Southern Biotech). Slides were developed with AEC substrate solution (Vector Laboratories) and then counterstained with hematoxylin. Images were acquired at x200 magnification using Aperio ScanScope digital scanners.
Cai Y., Xue F., Qin H., Chen X., Liu N., Fleming C., Hu X., Zhang H.G., Chen F., Zheng J, & Yan J. (2019). Differential Roles of the mTOR-STAT3 Signaling in Dermal γδ T Cell Effector Function in Skin Inflammation. Cell reports, 27(10), 3034-3048.e5.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!