Uas yki v5

Manufactured by Bloomington Drosophila Stock Center

The UAS-yki-V5 is a genetic tool used in Drosophila research. It consists of a UAS (Upstream Activating Sequence) promoter that drives the expression of the yki-V5 gene, which encodes the Yorkie protein with a V5 epitope tag. This tool allows for the targeted overexpression of the Yorkie protein in Drosophila cells and tissues.

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2 protocols using uas yki v5

Genetic Toolbox for Drosophila Research

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All crosses were maintained at 25 °C unless otherwise noted. Alleles used in these studies (Bloomington stock number indicated) are as follows: thread-lacZ (#12093), ex697 (ex-lacZ, #44248), e2f1rM729 (e2f1-lacZ, #34054), UAS-yki-V5 (#28819), yki^B5 (#36290), mirr^DE-Gal4 (#29650), EcRE-lacZ (#4517), Stat92E-10XGFP (#26198) (courtesy of R.Read), UAS-diap1 (#6657), UAS-p35 (#5073), UAS-dcr2;enGal4,UAS-GFP (#25752) obtained from the Bloomington Drosophila Stock Center. UAS-yki-IR (v104523) and UAS-ctp-IR (v43116) were obtained from the Vienna Drosophila Resource Center (VDRC). Other alleles used were ctp^ex3 (Gift of Bill Chia), enGal4/CyO, HRE-lacZ, HREmut6-lacZ, 2B2-lacZ, 2B2C-lacZ (all gift of D.J. Pan), brC12-lacZ (K. Irvine), GMR-Gal4, UAS-ykiS168A:GFP (K. Harvey), E-spl(m)ß-CD2, Su(H)-lacZ, PCNA-GFP, ban-3xGFP, and UAS-yki (D.J. Pan).

Barron D.A, & Moberg K. (2016). Inverse regulation of two classic Hippo pathway target genes in Drosophila by the dimerization hub protein Ctp. Scientific Reports, 6, 22726.

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Drosophila Genetic Toolkit for Research

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Stocks used in this study include the following: hsFLP; act<stop<Gal4, UAS-yki^S168A.v5, hsFLP;act<stop<Gal4, UAS-RFP^NLS, hsFLP, UAS-GFP^NLS; tub-Gal80, FRT40A (MARCM FRT 40A), ubi>RFP, FRT40A, banΔ1, banGFP, en-Gal4, dpp-lacZ, UAS-RFP, AP-1-RFP (53 (link)), and TIE-DYE (50 (link)). Stocks were obtained from the Bloomington Drosophila Stock Center (BDSC): UAS-yki^S168A.v5 (#28818), UAS-yki.v5 (#28819), UAS-yki::GFP (#28815), wts^X1 (#44251), smo³ (#3277), UAS-ci^RNAi (#64928), tara1-lacZ (#6403), UAS-tara^RNAi (#31634), nub-Gal4 (#42699), 30A-Gal4 (#37534), UAS-dMyc (#9674), UAS-rasv12 (#4847), and UAS-jnk^DN (#9311). Ci-lacZ was a gift from D. Kalderon (Columbia University, USA). UAS-bantam^sponge and UAS-bantam.D were gifts from S. Cohen (University of Copenhagen, Denmark). hs-Pc-sensor was a gift from V. Pirrotta (Rutgers University, USA). UAS-myc::tara was a gift from R. Smith-Bolton [University of Illinois, Urbana-Champaign, USA (originally from M. Cleary, University of California, Merced, USA)]. MARCM FRT19A and sd^47M, FRT19A were gifts from D. Pan.

Bairzin J.C., Emmons-Bell M, & Hariharan I.K. (2020). The Hippo pathway coactivator Yorkie can reprogram cell fates and create compartment-boundary–like interactions at clone margins. Science Advances, 6(50), eabe8159.

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