Milliplex human amyloid beta tau magnetic beadpanel hnabtmag 68k

Manufactured by Merck Group

Sourced in United States

The MILLIPLEX Human Amyloid Beta Tau Magnetic Bead Panel (HNABTMAG-68K) is a multiplex assay that allows for the simultaneous quantification of multiple analytes related to amyloid beta and tau proteins in human biological samples. The panel utilizes magnetic beads coated with specific capture antibodies to detect and measure the target analytes.

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Lab products found in correlation

Nf light elisa kit, by UmanDiagnostics (2 mentions) Luminex platform, by Merck Group (1 mentions)

2 protocols using milliplex human amyloid beta tau magnetic beadpanel hnabtmag 68k

Quantifying Neurodegeneration Biomarkers in CSF

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CSF samples were thawed in a biological safety cabinet just before analysis. To measure NFL concentration, a commercial sandwich enzyme‐linked immunosorbent assay (ELISA; NF‐light ELISA kit, Uman Diagnostics, Umeå, Sweden) was performed according to the manufacturer's instructions. Concentrations of Aβ40, Aβ42, p‐tau (pThr181), and t‐tau were determined using a MILLIPLEX Human Amyloid Beta Tau Magnetic BeadPanel (HNABTMAG‐68K, EMD Millipore, Billerica, MA, USA) completed on a Biorad Luminex platform according to the manufacturer's instructions. Within plate and interplate coefficients of variation were <5% and <15% for NFL; and <10% and <15% for the multiplex, respectively. Repeat samples collected from a given animal were run together on the same plate, except for two in Figure 2A (long delays).

Boehnke S.E., Robertson E.L., Armitage‐Brown B., Wither R.G., Lyra e Silva N.M., Winterborn A., Levy R., Cook D.J., De Felice F.G, & Munoz D.P. (2020). The effect of lumbar puncture on the neurodegeneration biomarker neurofilament light in macaque monkeys. Alzheimer's & Dementia : Diagnosis, Assessment & Disease Monitoring, 12(1), e12069.

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CSF Biomarker Quantification Protocol

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CSF samples were thawed in a biological safety cabinet just before analysis. To measure Aβ40, Aβ42, p‐tau (pThr181), and t‐tau, a MILLIPLEX Human Amyloid Beta Tau Magnetic Bead Panel (HNABTMAG‐68K, EMD Millipore) was completed on a Biorad Luminex platform according to the manufacturer's instructions. NfL was measured using a commercial sandwich ELISA (NF‐light ELISA kit, UmanDiagnostics) performed according to the manufacturer's instructions. Reference samples were included on all multiplex and NfL ELISA plates for comparison across plates. Within‐plate and interplate coefficients of variation were <15% for the multiplex; and <10% for NfL.

Robertson E.L., Boehnke S.E., Lyra e Silva N.D., Armitage‐Brown B., Winterborn A., Cook D.J., De Felice F.G, & Munoz D.P. (2022). Characterization of cerebrospinal fluid biomarkers associated with neurodegenerative diseases in healthy cynomolgus and rhesus macaque monkeys. Alzheimer's & Dementia : Translational Research & Clinical Interventions, 8(1), e12289.

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