Bax d2e11 rabbit mab

Manufactured by Cell Signaling Technology

Sourced in United States

Bax (D2E11) rabbit mAb is a laboratory reagent used for detecting the Bax (BCL2 Associated X, Apoptosis Regulator) protein. Bax is a pro-apoptotic member of the Bcl-2 protein family and plays a key role in the regulation of apoptosis (programmed cell death). The Bax (D2E11) rabbit mAb can be used in various applications, such as Western blotting, immunohistochemistry, and immunofluorescence, to study the expression and localization of the Bax protein in biological samples.

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3 protocols using bax d2e11 rabbit mab

Apoptosis Induction and Signaling Pathways

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CPS-C was obtained from Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College (Peking, China). Oxaliplatin was purchased from Sigma-Aldrich Co. (St. Louis, Missouri, USA). MTT was purchased from Sigma-Aldrich Co. (St. Louis, Missouri, USA). The Annexin V Apoptosis Detection kit was purchased from BD Biosciences (Franklin Lakes, New Jersey, USA). PI3 kinase p110α (C73F8) rabbit mAb (#4249), phospho-Akt (Ser473) (D9E) rabbit mAb (#4060), phospho-mTOR (Ser2448) (D9C2) rabbit mAb (#5536), phospho-mTOR (Ser2448) (49F9) rabbit mAb (IHC Specific) (#2976), Bcl-2 (124) mouse mAb (#15071), Bcl-xL (54H6) rabbit mAb (#2764), Bax (D2E11) rabbit mAb (#5023), and caspase-3 antibody (#9662) were purchased from Cell Signaling Technology (Danvers, Massachusetts, USA). Anti-GAPDH was purchased from Santa Cruz Biotechnology, Inc. (Dallas, Texas, USA). The In Situ Cell Death Detection Kit was purchased from Sigma-Aldrich Co. (St. Louis, Missouri, USA).

Shen Z., Xu L., Li J, & Zhang N. (2017). Capilliposide C Sensitizes Esophageal Squamous Carcinoma Cells to Oxaliplatin by Inducing Apoptosis Through the PI3K/Akt/mTOR Pathway. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 23, 2096-2103.

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Antibody and Chemical Reagents for Cancer Research

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B7-H3 (D9M2L) XP rabbit monoclonal antibody (mAb), Bcl-2(50E3) rabbit mAb, Bcl-xL (54H6) rabbit mAb, Bax (D2E11) rabbit mAb, EGF receptor (C74B9) (D9M2L) rabbit mAb, IκB-alpha (44D4) rabbit mAb, ERK rabbit mAb, p-ERK rabbit mAb and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) rabbit mAb were purchased from Cell Signaling Technology (USA). 4H7(Jiangsu Key Laboratory of Clinical Immunology, Soochow University, Suzhou). The horseradish peroxidase–conjugated secondary anti-rabbit antibodies were purchased from Bio-Rad (CA, USA). Gemcitabine was purchased from Lilly France, Inc. (Neuilly, France). A Phosphoprotein Purification Kit was purchased from Qiagen Sample and Assay Technologies (Germany). Dulbecco’s modified Eagle’s Medium (DMEM) was purchased from Sigma–Aldrich (MO, USA). Fetal bovine serum (FBS) was supplied by Sijiqing Biological Manufacturer Co., Ltd (Hangzhou, China). The Annexin V–fluorescein isothiocyanate (FITC) Apoptosis Detection Kit was purchased from Miltenyi Biotec (Germany).

Li D., Wang J., Zhou J., Zhan S., Huang Y., Wang F., Zhang Z., Zhu D., Zhao H., Li D., Chen G., Zhu X, & Zhao X. (2017). B7-H3 combats apoptosis induced by chemotherapy by delivering signals to pancreatic cancer cells. Oncotarget, 8(43), 74856-74868.

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Investigating Apoptosis Signaling Pathways

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Roswell Park Memorial Institute (RPMI) 1640 medium, Dulbecco’s Modified Eagle’s Medium (DMEM) medium, penicillin/streptomycin, fetal bovine serum (FBS), phosphate-buffered saline (PBS), L-glutamine, and trypsin-EDTA were purchased from Gibco (Grand Island, NY, USA). Dimethyl sulfoxide (DMSO), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT), propidium iodide (PI), Hoechst 33342, cisplatin, etoposide, doxorubicin, and bovine serum albumin (BSA) were purchased from Sigma-Aldrich, Co. (St. Louis, MO, USA). The primary antibodies used in the experiments were β-Actin (13E5) rabbit mAb (Cat#4970, RRID: AB_2223172), PARP (46D11) rabbit mAb (Cat# 9532, RRID: AB_2160739), Mcl-1 (D2W9E) rabbit mAb (Cat#94296, RRID: AB_2722740), Bcl-2 (D55G8) rabbit mAb (Cat# 4223, RRID: AB_1903909), and Bax (D2E11) rabbit mAb (Cat#5023, RRID: AB_10557411) which were obtained from Cell Signaling Technology (Danvers, MA, USA). The respective secondary antibodies, anti-rabbit IgG, HRP-linked antibody (Cat#7074, RRID: AB_2099233) was also purchased from Cell Signaling Technology (Danvers, MA, USA). Moreover, goat anti-rabbit IgG H&L (Alexa Fluor 488, Cat#ab150077, RRID: AB_2630356) was obtained from Abcam (Cambridge, MA, USA).

Petsri K., Yokoya M., Tungsukruthai S., Rungrotmongkol T., Nutho B., Vinayanuwattikun C., Saito N., Takehiro M., Sato R, & Chanvorachote P. (2020). Structure–Activity Relationships and Molecular Docking Analysis of Mcl-1 Targeting Renieramycin T Analogues in Patient-derived Lung Cancer Cells. Cancers, 12(4), 875.

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