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Mouse mab against vinculin

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The Mouse mAb against vinculin is a monoclonal antibody that specifically binds to the vinculin protein. Vinculin is a cytoskeletal protein involved in cell-cell and cell-matrix adhesion. This antibody can be used for the detection and localization of vinculin in various applications, such as Western blotting, immunohistochemistry, and immunocytochemistry.

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Lab products found in correlation

Hrp conjugated anti mouse or anti rabbit igg, by Santa Cruz Biotechnology (1 mentions) Alexa fluor 594 conjugated phalloidin, by Thermo Fisher Scientific (1 mentions) E cadherin, by BD (1 mentions) α catenin, by BD (1 mentions) Bcl xl, by Santa Cruz Biotechnology (1 mentions) N cadherin, by BD (1 mentions) Mabs against e cadherin, by BD (1 mentions) Rat mab against ha, by Roche (1 mentions) Mouse mab against vimentin, by Zymo Research (1 mentions)

Close spelling variants of this product

Vinculin (439 citations) Vinculin (mouse, (2 citations)

3 protocols using mouse mab against vinculin

1

Antibodies for Cell Signaling Analysis

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The specific antibody against ARHGEF11exon38(+) isoform was raised using a peptide coded by exon38, SKVVPALPESGQSEP, as an antigen. Mouse mAbs against RhoA, Rac1, CDC42, E-cadherin, α-catenin, β-catenin, and p27 were obtained from BD Biosciences; rabbit Abs against p-EGFR, EGFR, p-Erk1/2, Erk1/2, p-p38, p38, cyclin-D1, cyclin-E1, cyclin-A2, cyclin-B1, ARHGEF2, keratin8/18, and vimentin were from Cell signaling; rabbit pAb against N-cadherin was from EMD; rabbit Abs against ARHGEF18, Gα13, p15, and Ki67 were from Genetex; rabbit Abs against ZO-1, ZO-2, ZO-3, occludin, claudin-3, and claudin-4 were from Invitrogen; mouse mAbs against ARHGEF11, Bcl-2, and Bcl-xL were from Santa Cruz Biotech.; mouse mAb against vinculin was from Sigma; mouse mAbs against GAPDH, GFP, and Myc were from Wako; Alexa Fluor 488/594-conjugated anti-mouse or anti-rabbit IgG, and Alexa Fluor 594-conjugated phalloidin were from Invitrogen; HRP-conjugated anti-mouse or anti-rabbit IgG were from Santa Cruz Biotech.
Itoh M., Radisky D.C., Hashiguchi M, & Sugimoto H. (2017). The exon 38-containing ARHGEF11 splice isoform is differentially expressed and is required for migration and growth in invasive breast cancer cells. Oncotarget, 8(54), 92157-92170.
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2

Antibody Immunodetection Protocol

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Mouse monoclonal antibodies (mAbs) against E-cadherin, N-cadherin, p120, Laminin5, and fibronectin were purchased from BD Biosciences (Lexington, KY, USA). Mouse mAb against vimentin and rabbit polyclonal antibody against occludin were obtained from Zymed Laboratories (South San Francisco, CA, USA). Mouse mAb against Snail was purchased from Cell Signaling Technology (Danvers, MA, USA). Mouse mAb against vinculin was purchased from Sigma-Aldrich (St Louis, MO, USA). Rat mAb against HA was purchased from Roche Applied Science (Mannheim, Germany). All secondary antibodies were purchased from Jackson ImmunoResearch Laboratories (West Grove, PA, USA).
Tanaka S., Kobayashi W., Haraguchi M., Ishihata K., Nakamura N, & Ozawa M. (2016). Snail1 expression in human colon cancer DLD-1 cells confers invasive properties without N-cadherin expression. Biochemistry and Biophysics Reports, 8, 120-126.
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3

Antibodies for Cell-Cell Adhesion Analysis

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Mouse monoclonal antibodies (mAbs) against E-cadherin, β-catenin, γ-catenin, fibronectin, and p120-catenin were purchased from Transduction Laboratories (Lexington, KY). A mouse mAb against vinculin and a rat mAb against HA were bought from Sigma (St. Louis, MO) and Roche Diagnostics GmbH (Mannheim, Germany), respectively. A rabbit mAb against Slug was acquired from Cell Signaling Technology Japan (Tokyo, Japan). A goat polyclonal antibody against ZEB1 was purchased from Santa Cruz Biotechnology (Santa Cruz, CA). All secondary antibodies were acquired from Jackson ImmunoResearch Laboratories (West Grove, PA).
Kobayashi W, & Ozawa M. (2018). The epithelial-mesenchymal transition induced by transcription factor LEF-1 is independent of β-catenin. Biochemistry and Biophysics Reports, 15, 13-18.
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