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Spark fluorometric plate reader

Manufactured by Tecan
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The Spark fluorometric plate reader is a versatile laboratory instrument used for the detection and quantification of fluorescent signals in microplate-based assays. It provides reliable and accurate measurements of fluorescence intensity, making it a valuable tool for a wide range of applications in life science research, drug discovery, and clinical diagnostics.

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Lab products found in correlation

Amplex red cholesterol fluorometric detection assay, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

Spark plate reader (292 citations) Spark reader (36 citations) Fluorometric plate reader (3 citations)

2 protocols using spark fluorometric plate reader

1

Quantification of Cellular Cholesterol Levels

Check if the same lab product or an alternative is used in the 5 most similar protocols
An Amplex Red cholesterol fluorometric detection assay (Thermo-Fischer, A12216) was used for quantification of total cholesterol levels in cell culture. Cells were cultured as for immunostaining, treated as indicated in experimental set-up for a total of 72 h. For collection of samples, cell media was removed, and cells rinsed with PBS. Lipids were extracted with a solution of chloroform:methanol (at a ratio of 1:2) for 30+ minutes at 4 °C. Extraction buffer was collected and centrifuged at 10,000 rpm for 10 min at 4 °C to pellet any remaining cell debris; supernatants were collected and stored at −80 °C until assay could be performed. Amplex Red assay was carried out according to manufacturer instructions, with samples diluted 1:1 (a total of 25 μL from each well used per reaction) with 1X reaction buffer. Assay reaction was carried out on a 96 well plate, which was read using a Tecan Spark fluorometric plate reader for quantification.
Triplet E.M., Kim H.N., Yoon H., Radulovic M., Kleppe L., Simon W.L., Choi C.I., Walsh P.J., Dutton J.R, & Scarisbrick I.A. (2021). The thrombin receptor links brain derived neurotrophic factor to neuron cholesterol production, resiliency and repair after spinal cord injury. Neurobiology of disease, 152, 105294.
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2

Quantification of Cellular Cholesterol Levels

Check if the same lab product or an alternative is used in the 5 most similar protocols
An Amplex Red cholesterol fluorometric detection assay (Thermo-Fischer, A12216) was used for quantification of total cholesterol levels in cell culture. Cells were cultured as for immunostaining, treated as indicated in experimental set-up for a total of 72 h. For collection of samples, cell media was removed, and cells rinsed with PBS. Lipids were extracted with a solution of chloroform:methanol (at a ratio of 1:2) for 30+ minutes at 4 °C. Extraction buffer was collected and centrifuged at 10,000 rpm for 10 min at 4 °C to pellet any remaining cell debris; supernatants were collected and stored at −80 °C until assay could be performed. Amplex Red assay was carried out according to manufacturer instructions, with samples diluted 1:1 (a total of 25 μL from each well used per reaction) with 1X reaction buffer. Assay reaction was carried out on a 96 well plate, which was read using a Tecan Spark fluorometric plate reader for quantification.
Triplet E.M., Kim H.N., Yoon H., Radulovic M., Kleppe L., Simon W.L., Choi C.I., Walsh P.J., Dutton J.R, & Scarisbrick I.A. (2021). The thrombin receptor links brain derived neurotrophic factor to neuron cholesterol production, resiliency and repair after spinal cord injury. Neurobiology of disease, 152, 105294.
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