Ab155421

Manufactured by Abcam

Sourced in United States

Ab155421 is a lab equipment product from Abcam. It is designed for use in scientific research applications.

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Lab products found in correlation

3 protocols using ab155421

Comprehensive Analysis of Hepatic Transporters

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HB (collection in Sichuan, 20042901), ZZ (collection in Fujian, 210303), DH (collection in Gansu, 20201211), and MX (collection in Jiangsu, 21032501) were obtained from Simcare Ltd. Primary antibodies against ZO-1 (21773-1-AP, 1:1000), occludin (13409-1-AP, 1:1000), claudin-1 (13050-1-AP, 1:1000), and the second antibody of beta-actin (20536-1-AP, 1:10000) were purchased from Proteintech Group Inc. Primary antibodies against NTCP (ab131084, 1:1000), BSEP (ab155421, 1:1000), CYP7A1 (ab234982, 1:1000), MRP2 (ab172630, 1:1000), and FXR1 (ab129089, 1:1000) were obtained from Abcam Inc. ANIT (N106389), UDCA(U110695), and olive oil (O108685) were purchased from the Aladdin Chemical Reagent Co. Dimethyl sulfoxide (DMSO) was purchased from Sigma-Aldrich (reference D4540) (St-Louis, MO, USA). The TRIzol total RNA extraction kit was obtained from Life Technologies.

Wang W., Jiang S., Xu C., Tang L., Liang Y., Zhao Y, & Zhu G. (2022). Transcriptome and Gut Microbiota Profiling Analysis of ANIT-Induced Cholestasis and the Effects of Da-Huang-Xiao-Shi Decoction Intervention. Microbiology Spectrum, 10(6), e03242-22.

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Immunohistochemistry Imaging of Zebrafish Liver

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At 5–7 dpf, zebrafish were anesthetized in 0.1% tricaine and fixed in 10% formalin overnight at room temperature (RT), and then embedded in paraffin blocks and sectioned at 2–4 nm thickness. Slides were dewaxed through 2 changes of xylene and hydrated through 100%, 90%, and 70% ethanol. Antigen retrieval was performed using a pressure cooker, and then slides were treated with H₂O₂ and methanol for peroxidase inhibition. Blocking was performed using 10% goat serum in PBS plus 0.1% Tween 20 (PBS-T) for 1 hour at RT, and then slides were incubated with a primary antibody for 1 hour, washed for 15 minutes in PBS-T, and incubated 30 minutes in secondary antibody. Slides were mounted using Prolong Diamond and cured at RT for 48 hours before proceeding to imaging on a Leica SP8 Lightning confocal microscope. The following antibodies were used: Mdr1 (Thermo Fisher Scientific, BS-0563R, 1:100); BSEP (MilliporeSigma, HPA019035, 1:100); BSEP (Abcam, ab155421, 1:200); GFP (Abcam, ab252881, 1:100); Rab11 (Genetex, GTX127328, 1:300); and 2F-11 (Abcam, ab71286, 1:100).

Karolczak S., Deshwar A.R., Aristegui E., Kamath B.M., Lawlor M.W., Andreoletti G., Volpatti J., Ellis J.L., Yin C, & Dowling J.J. (2023). Loss of Mtm1 causes cholestatic liver disease in a model of X-linked myotubular myopathy. The Journal of Clinical Investigation, 133(18), e166275.

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Western Blot Protocol for Protein Detection

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Whole cell lysates were derived from cells or tissues using radioimmunoprecipitation assay (RIPA) lysis buffer. Proteins (20–40 μg) with SDS-loading buffer were loaded onto gels and run on SDS-PAGE; then, proteins were transferred to the polyvinylidene fluoride (PVDF) membrane. The PVDF membranes were incubated with primary antibodies against Pro-caspase-1 (1:2000, #ab179515, Abcam), caspase-1 (1:2000, #ab108362, Abcam), NLRP3 (1:2000, #ab16097, Abcam), IL-1β (1:1000, #AF-401-NA, R&D), HO-1 (1:500, #sc-10789, Santa Cruz), GAPDH (1:500, #sc-47724, Santa Cruz), β-actin (1:500, #sc-81178, Santa Cruz), Tubulin (1:1000, #556321, BD Pharmingen), FXRa (1:2000, #ab187735, Abcam), Cyp8b1 (1:2000, #ab175843, Abcam), Cyp27a1 (1:1000,#ab126785, Abcam), Sult2a1 (1:500, #sc-376629, Santa Cruz), Bsep (1:2000, #ab155421, Abcam), Mrp3 (1:500, #sc-5776, Santa Cruz), Ntcp (1:2000, #ab131084, Abcam), and BAAT (1:2000, #ab83882, Abcam). Then, appropriate horseradish peroxidase (HRP)-linked secondary antibodies were incubated for further 1 hour at room temperature. Luminal and H₂O₂ substrates were used for chemiluminescence detection.

Chen W., Ding M., Ji L., Yao J., Guo Y., Yan W., Yu S., Shen Q., Huang M., Zheng Y., Lin Y., Wang Y., Liu Z., Lu L, & Jin X. (2023). Bile acids promote the development of HCC by activating inflammasome. Hepatology Communications, 7(9), e0217.

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