Fitc mouse anti human cd45

Manufactured by BioLegend

The FITC mouse anti-human CD45 is a fluorescently labeled antibody that binds to the CD45 antigen expressed on human leukocytes. CD45 is a transmembrane protein tyrosine phosphatase that plays a crucial role in the activation and regulation of T and B cells.

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Lab products found in correlation

Pe mouse anti human cd29, by Thermo Fisher Scientific (1 mentions) Hematoxylin eosin staining kit, by Solarbio (1 mentions) Pe mouse igg1 κ isotype control, by BioLegend (1 mentions) Fitc mouse igg1 κ isotype control, by BioLegend (1 mentions) Apc mouse igg1 κ isotype control, by BioLegend (1 mentions) Elisa kit, by Solarbio (1 mentions) Gelred dye, by Biotium (1 mentions) Depc treated water, by Solarbio (1 mentions) Rna loading buffer, by Takara Bio (1 mentions) Tgf β1, by Thermo Fisher Scientific (1 mentions) Superreal premix plus sybr green, by Tiangen Biotech (1 mentions) Cck 8, by Beyotime (1 mentions) Rnasimple total rna kit, by Tiangen Biotech (1 mentions) Edu staining kit, by Beyotime (1 mentions) 70 μm filter, by BD (1 mentions) Facsaria 2 cell sorter, by BD (1 mentions) Collagenase, by Worthington (1 mentions) Dispase, by STEMCELL (1 mentions)

3 protocols using fitc mouse anti human cd45

Surface Antigen Characterization of Cells

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To perform surface antigens staining, cells were incubated with FITC mouse anti-human CD45 (1:20; #13234199; Biolegend), FITC mouse anti-human CD19 (1:20; #4310183; ebioscience), FITC mouse anti-human CD34 (1:20; #4330486; Invitrogen), PE mouse anti-human CD90 (1:20; #4303050; ebioscience), PE mouse anti-human CD29 (1:20; #4303570; ebioscience) or PE mouse anti-human CD105 (1:20; #4300023; ebioscience) antibody at 4 °C for 30 min.

Chen B., Cai T., Huang C., Zang X., Sun L., Guo S., Wang Q., Chen Z., Zhao Y., Han Z., Xu R., Xu W., Wang M., Shen B, & Zhu W. (2021). G6PD-NF-κB-HGF Signal in Gastric Cancer-Associated Mesenchymal Stem Cells Promotes the Proliferation and Metastasis of Gastric Cancer Cells by Upregulating the Expression of HK2. Frontiers in Oncology, 11, 648706.

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Mesenchymal Stem Cell Characterization

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TGF-β1 (PEPRO TECH, China, 100-21-10 μg), PC-MSC (Provided by Shenzhen 150 Biomedical Co.,Ltd.), ESC (Provided by ATCC), FITC Mouse IgG1 κ Isotype control (Biolegend, 7313762), PE Mouse IgG1 κ Isotype control (Biolegend, 9217868), APC Mouse IgG1 κ Isotype control (Biolegend, 9282592), PE Mouse Anti-Human CD73 (Biolegend, 8151901), APC Mouse Anti-Human CD105 (Biolegend, 9277133), FITC Mouse IgG2a κ Isotype control (Biolegend, 8241935), FITC Mouse Anti-Human CD14 (Biolegend, 8299630), FITC Mouse Anti-Human CD45 (Biolegend, 8206704), DMEM/F12 medium (Peiyuan, Shanghai, L310 kJ), Fetal bovine serum (Gemini, 900-108), Trypsin digestive fluid (Beyo, C0203), DPBS (Beyo, C0221G), CCK8 (Beyotime, C0039), EDU staining kit (Beyotime, C0081s), Hematoxylin-eosin staining kit (Solarbio, G1120), DEPC-treated water (Solarbio, R1600), trichloromethane (Sinopharm Chemical Reagent Co. LTD., 10006818), GelRed dye (Biotium, #41003), RNA Loading Buffer (TaKaRa, 9168), Tris-Borate-EDTA Buffer (TBE) 10× Powder, pH8.3 (TaKaRa, T9122), RNAsimple Total RNA Kit (TIANGEN, DP419), All-in-One First-Strand Synthesis Master Mix (Yugong Biolabs, EG15133S), SuperReal PreMix Plus (SYBR Green) (TIANGEN, FP205-02), and Methacrylate anhydride (MAA, 0.1 mL/1 g gelatin), ELISA Kit (Solarbio, SEKH-0052), MateRegen^® Gel (2104006, BioRegen Biomedical (Changzhou) Co., Ltd.).

Zhu Y., Wang T., Bao M.J., Qu X.H, & Li Z.M. (2023). Effect of stem cell conditional medium-loading adhesive hydrogel on TGF-β1-induced endometrial stromal cell fibrosis. Frontiers in Bioengineering and Biotechnology, 11, 1168136.

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Isolation and Culture of Shoulder Muscle Stem Cells

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Muscle biopsies were obtained from arthroscopic shoulder surgeries on patients with varying rotator cuff tear states. Samples from deltoid, supraspinatus, and infraspinatus muscles were procured. Samples were digested using 0.25% collagenase (Worthington Biochemical) and dispase (Stem Cell Technologies) for 30 min at 37°C, before being minced and digested for a subsequent 10 min. Cells were passed through a 70 μm filter (BD) and centrifuged at 2000 RPM for 10 min at 4°C. Cells were then resuspended in FACS buffer (2.5% normal goat serum and 1 mM EDTA in PBS) and stained using PE mouse anti human NCAM (BD 561903), eFluor450 mouse anti human CD31 (eBioscience 48-0319-42), and FITC mouse anti-human CD45 (BioLegend 304017) for 20 min on ice. Cells were centrifuged at 2000 rpm for 4 min, resuspended in FACS buffer, and sorted using a FACSAria 2 cell sorter (BD). Following sorting, SMPs were kept in 20% FBS in one well of a 24-well plate and passaged when confluent. Medium was changed every other day.

Thomas K.A., Gibbons M.C., Lane J.G., Singh A., Ward S.R, & Engler A.J. (2016). Rotator Cuff Tear State Modulates Self-Renewal and Differentiation Capacity of Human Skeletal Muscle Progenitor Cells. Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 35(8), 1816-1823.

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