Log In Sign up
The largest database of trusted experimental protocols

Apc conjugated cd45 30 f11

Manufactured by Thermo Fisher Scientific
Visit Supplier
Sourced in United States

APC-conjugated CD45 (30-F11) is a fluorescently-labeled antibody that binds to the CD45 antigen, which is expressed on the surface of various hematopoietic cells. The APC (Allophycocyanin) fluorescent label allows for detection and analysis of CD45-positive cells using flow cytometry.

Automatically generated - may contain errors

Lab products found in correlation

Lsrii flow cytometer, by BD (1 mentions) Apc conjugated cd25 pc61, by BD (1 mentions) Lsrfortessa, by BD (1 mentions) F4 80 bm8, by BioLegend (1 mentions) Cd19 6d5, by BioLegend (1 mentions) Gr 1 rb6 8c5, by BioLegend (1 mentions) Amnis imagestream x mark 2, by Merck Group (1 mentions) Cd11b m1 70, by BioLegend (1 mentions) Cd41 mwreg30, by BioLegend (1 mentions)

Close spelling variants of this product

CD45-APC (69 citations) CD45 (30-F11, (34 citations) APC-conjugated CD45 (5 citations)

2 protocols using apc conjugated cd45 30 f11

1

Lymphocyte Immunophenotyping in Spinal Cord

Check if the same lab product or an alternative is used in the 5 most similar protocols
Lymphocytes isolated from the spinal cord were immunophenotyped with fluorescent antibodies (1:200) for the following cell-surface markers: fluorescein isothiocyanate (FITC)-conjugated CD4 (GK1.5; BD Biosciences), PE-Cy7-conjugated CD8 (Ly-2; BD Biosciences), antigen-presenting cell (APC)-conjugated CD25 (PC61; BD Biosciences), APC-conjugated CD45 (30-F11; eBioscience), FITC-conjugated F4/80 (Ci-A3-1; Serotech), and Alexa Fluor 647-conjugated FOXP3 (FJK-16 s; eBioscience). PE-conjugated I-Ab/M133–147 tetramers and PE-conjugated Db/S510–518 (8 μg/ml; obtained from the National Institutes of Health/National Institute of Allergy and Infectious Diseases MHC Tetramer Core Facility) were used to stain viral-specific CD4 T cells and viral-specific CD8 T cells, respectively. Appropriate isotype controls were used for each antibody. Cells were run on a LSRII flow cytometer (BD Biosciences) and analyzed with FlowJo software (Tree Star). All cells for flow cytometry were blocked with anti-mouse CD16/32 (Mouse Fc Block; 1:200; BD Biosciences) for 20 min at 4°C.
Chen L., Coleman R., Leang R., Tran H., Kopf A., Walsh C.M., Sears-Kraxberger I., Steward O., Macklin W.B., Loring J.F, & Lane T.E. (2014). Human Neural Precursor Cells Promote Neurologic Recovery in a Viral Model of Multiple Sclerosis. Stem Cell Reports, 2(6), 825-837.
+ Open protocol
+ Expand
2

Multi-Color Flow Cytometry Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Dissociated cells were incubated with the following antibodies (1:500) in Flow Cytometry Staining Buffer on ice for 30 minutes. Allophycocyanin (APC)-conjugated CD45 (30F-11, eBioscience [San Diego, CA, USA] 17–0451-82), phycoerythrin (PE)-conjugated CD48 (HM48-1, BioLegend [San Diego, CA, USA] 103405), CD41 (MWReg30, BioLegend 133905), CD79b (HM79-12, BioLegend 132803), F4/80 (BM8, BioLegend 123109), Gr-1 (RB6-8C5, BioLegend 108407), brilliant violet 650 (BV650)-conjugated CD150 (TC15-12F12.2, BioLegend 115931), CD19 (6D5, BioLegend 115541), CD11b (M1/70, BioLegend 101239). Flow cytometry analysis was performed using a four-laser BD LSR Fortessa (Ex. 405/488/561/640 nm) and FACSDiva software. Acquired raw data were further analyzed on FlowJo software (TreeStar, Ashland, OR, USA). Representative plots of at least three independent biological samples are shown in the figures. ImageStream analysis was performed using Amnis ImageStreamX Mark II (MilliporeSigma, Burlington, MA, USA). Acquired raw data were further analyzed on Amnis IDEAS software.
Matsushita Y., Chu A.K., Ono W., Welch J.D, & Ono N. (2021). Intercellular Interactions of an Adipogenic CXCL12-Expressing Stromal Cell Subset in Murine Bone Marrow. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 36(6), 1145-1158.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!