Bafilomycin a1 b1793 2ug

Manufactured by Merck Group

Bafilomycin A1 (B1793-2UG) is a potent and selective inhibitor of vacuolar-type H+-ATPase (V-ATPase). It is a macrolide antibiotic isolated from various Streptomyces species. This compound is frequently used as a research tool to study the role of V-ATPase in cellular processes.

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Lab products found in correlation

Torin1 cay10997, by Cayman Chemical (2 mentions) Phospho thr389 p70s6k sc 11759, by Santa Cruz Biotechnology (2 mentions) Total ulk1 antibody, by Cell Signaling Technology (2 mentions) Chloroquine c6628, by Merck Group (1 mentions) Beclin 1 antibody, by Cell Signaling Technology (1 mentions) Lc 3 antibody nb100 2220, by Novus Biologicals (1 mentions) Total s6 antibody, by Cell Signaling Technology (1 mentions) β tubulin, by Merck Group (1 mentions) β actin antibody, by Merck Group (1 mentions) Filipin 3, by Cayman Chemical (1 mentions) Apilimod, by MedChemExpress (1 mentions) Lc3 nb100 2220, by Novus Biologicals (1 mentions) Gsk2578215a, by Bio-Techne (1 mentions) Mjff2, by Abcam (1 mentions) Mli 2, by Bio-Techne (1 mentions) Lrrk2 in 1, by Bio-Techne (1 mentions) β actin, by Merck Group (1 mentions) P62 610833, by BD (1 mentions)

Close spelling variants of this product

Bafilomycin A1 (B1793) Bafilomycin A1 B1793 Bafilomycin

3 protocols using bafilomycin a1 b1793 2ug

Evaluation of LRRK2 and VPS34 Inhibitors

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The LRRK2in1 compound and the VPS34in1 were purchased from the Division of Signal Transduction Therapy, School of Life Sciences, and University of Dundee, UK. The GSK2578215A compound was purchased from Tocris. Bafilomycin A1 (B1793-2UG), Chloroquine (C6628) and Wortmannin (W3144-250UL) were purchased from Sigma-Aldrich. Torin-1 (CAY10997) was purchased from Cayman Chemicals.
Antibodies used were as follows: LC3 antibody (NB100-2220, Novus Biologicals); LRRK2 antibodies (MJFF#2, 3514-1/ab133474, Epitomics); total S6 antibody (2317, Cell Signalling); phospho Ser235/236 S6 antibody (2211S, Cell Signalling); total P70S6K antibody (sc-8418, Santa Cruz); phospho Thr389 P70S6K (sc-11759, Santa Cruz); total ULK1 antibody (8054 and 4773, Cell Signalling); phospho Ser757 ULK1 antibody (6888, Cell Signalling); Beclin-1 antibody (3738, Cell Signalling); β-actin antibody (A1978, Sigma Aldrich); β-tubulin (T6199, Sigma Aldrich).

Manzoni C., Mamais A., Roosen D.A., Dihanich S., Soutar M.P., Plun-Favreau H., Bandopadhyay R., Hardy J., Tooze S.A., Cookson M.R, & Lewis P.A. (2016). mTOR independent regulation of macroautophagy by Leucine Rich Repeat Kinase 2 via Beclin-1. Scientific Reports, 6, 35106.

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Vacuolin-1 and Apilimod Inhibit Lysosome Function

Cited 1 time

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Vacuolin-1 (18 (link)) was custom synthesized; Apilimod (HY-14644) was from MedChem Express, IN1 was a kind gift from N. Gray, Dana-Farber Cancer Institute and Harvard Medical School, Boston, MA (33 (link)), U-18666A (10009085) and Filipin III (70440) were from Cayman Chemical, Bafilomycin A1 (B1793-2UG) was from Sigma-Aldrich, Cycloheximide (239764) was from Calbiochem, and wheat germ agglutinin conjugated with Alexa Fluor-647 (W32466) was from ThermoFisher.

Kang Y.L., Chou Y.Y., Rothlauf P.W., Liu Z., Soh T.K., Cureton D., Case J.B., Chen R.E., Diamond M.S., Whelan S.P, & Kirchhausen T. (2020). Inhibition of PIKfyve kinase prevents infection by Zaire ebolavirus and SARS-CoV-2. Proceedings of the National Academy of Sciences of the United States of America, 117(34), 20803-20813.

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LRRK2 inhibitor-mediated autophagy regulation

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Chemical compounds were purchased as follows: LRRK2in1 [24 (link)] from the Division of Signal Transduction Therapy, School of Life Sciences, University of Dundee, U.K.; GSK2578215A and MLi-2 from Tocris [25 (link)]; bafilomycin-A1 (B1793-2UG) and cycloheximide (01810-1G) from Sigma-Aldrich; torin-1 (CAY10997) from Cayman Chemicals. Antibodies used were as follows: LC3 (NB100-2220, Novus Biologicals); total P70S6K (sc-8418, Santa Cruz); phospho Thr389 P70S6K (sc-11759, Santa Cruz); p62 (610833, BD Transduction Labs); total ULK1 antibody (8054 and 4773, Cell Signalling); phospho Ser757 ULK1 antibody (6888 and D7O6UCell Signalling); phospho Ser555 ULK1 antibody (5869, Cell Signalling); total LRRK2 antibody (MJFF2, Abcam); phosphor Ser935 LRRK2 antibody (UDD2, Abcam) and β-actin (A1978, Sigma-Aldrich). AMPK activator (A769662) was kindly provided by Dr MPMS.

Manzoni C., Mamais A., Dihanich S., Soutar M.P., Plun-Favreau H., Bandopadhyay R., Abeti R., Giunti P., Hardy J., R. Cookson M., Tooze S.A, & Lewis P.A. (2018). mTOR independent alteration in ULK1 Ser758 phosphorylation following chronic LRRK2 kinase inhibition. Bioscience Reports, 38(2), BSR20171669.

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