The single-stranded DNA library and primers were synthesized by Bioneer (Daejeon, Korea). pfu DNA polymerase was purchased from Biofact (Daejeon, Korea). Restriction enzymes and DNA ligases were purchased from Takara Bio (Shiga, Japan). Reduced graphene oxide was purchased from Graphene Supermarket (Calverton, NY, USA). Other chemicals were obtained from commercial sources with high quality.
Pfu dna polymerase
Manufactured by Biofact
Pfu DNA Polymerase is a thermostable DNA polymerase enzyme derived from the thermophilic archaeon Pyrococcus furiosus. It has a high fidelity and exhibits 3'-5' exonuclease proofreading activity, which enhances its ability to accurately replicate DNA.
Automatically generated - may contain errors
4 protocols using pfu dna polymerase
1
Graphene-based DNA Library Synthesis
2
PRNP Gene SNP Amplification via PCR
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PCR was performed to amplify fragments containing the E200K and V203I SNPs of the human PRNP gene with Pfu DNA Polymerase (BIOFACT, Daejeon, Korea). The experimental conditions of each primer for PCR are described in Table 3 . The PCR mixture contained 10 pmol of each primer, 2.5 µL of 10 × Taq DNA polymerase buffer, 1 μL of 10 mM dNTP mixture and 2.5 units of Taq DNA polymerase.
3
PRNP Gene SNP Amplification via PCR
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PCR was performed to amplify fragments containing the E200K and V203I SNPs of the human PRNP gene with Pfu DNA Polymerase (BIOFACT, Daejeon, Korea). The experimental conditions of each primer for PCR are described in Table 3 . The PCR mixture contained 10 pmol of each primer, 2.5 µL of 10 × Taq DNA polymerase buffer, 1 μL of 10 mM dNTP mixture and 2.5 units of Taq DNA polymerase.
4
Influenza A Virus Subtype Binding Assay
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Template (containing 30 random bases), primers, and all oligonucleotides were synthesized by Bioneer (Daejeon, Korea). Pfu DNA polymerase was purchased from Biofact (Daejeon, Korea), and restriction enzymes and DNA ligases were purchased from Takara Bio (Shiga, Japan). Chicken red-blood cells (RBCs), packed at 5%, were purchased from Innovative Research (Detroit, MI, USA). Allantoic fluid and influenza A virus subtypes (H5N1 (A/wild duck/Korea/SNU50-5/2011), H1N1 (A/Puerto Rico/8/1934), H9N2 (A/Duck/Hong Kong/702/1979), recombinatnt H5N1(p), and recombinant H5N6) were provided by the Seoul National University, College of Veterinary Medicine. M13 phage was purchased from New England Biolabs (Ipswich, MA, USA). Reduced graphene oxide (rGO) was purchased from Graphene Supermarket (Ipswich, MA, USA).
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