We obtained blood from HLA-A*11:01 positive healthy donors. PBMCs were isolated from peripheral blood with a density gradient technique (Ficoll, Axis-Shield) and cultured in RPMI 1640 supplemented with 10% human serum (Access Biologicals LLC) and 50 U/mL IL-2. NK cells were enriched from peripheral blood with RosetteSep™ Human NK Cell Enrichment Cocktail (Stemcell, 15,025) and cultured in RPMI 1640 supplemented with 10% human serum.
Human serum
Manufactured by STEMCELL
Sourced in Italy
Human serum is a cell culture reagent derived from the blood of healthy human donors. It provides a natural source of proteins, growth factors, and other components that support the growth and maintenance of cells in vitro.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using human serum
1
Isolation and Expansion of NK Cells
2
Ovarian Tissue Cryopreservation for Cancer Patients
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The Ethics Committee of S. Orsola-Malpighi Hospital of Bologna (74/2001/O) approved the study on human ovarian tissue. Ovarian tissues were obtained from three patients suffering from non-gynecological diseases (mean age ± S.D.: 28 ± 2 years) undergoing ovarian tissue cryopreservation to preserve their ovarian function before receiving anticancer therapies. All women donated their tissue for research purpose.
The ovarian biopsy was retrieved by laparoscopy and immediately transferred to the laboratory in phosphate buffered saline (PBS) medium (Stemcell Technologies, Milan, Italy) supplemented with 10% human serum (provided by the Transfusion Center of S.Orsola-Malpighi Hospital, Bologna, Italy) at 4°C. The medulla of the biopsy was removed using a surgical scissor, and the cortical tissue was dissected in strips (±1 cm × 2 mm × 1 mm), and slowly frozen according to the protocol previously described [35 (link)].
The ovarian biopsy was retrieved by laparoscopy and immediately transferred to the laboratory in phosphate buffered saline (PBS) medium (Stemcell Technologies, Milan, Italy) supplemented with 10% human serum (provided by the Transfusion Center of S.Orsola-Malpighi Hospital, Bologna, Italy) at 4°C. The medulla of the biopsy was removed using a surgical scissor, and the cortical tissue was dissected in strips (±1 cm × 2 mm × 1 mm), and slowly frozen according to the protocol previously described [35 (link)].
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