The antimicrobial susceptibility test was done with the disk diffusion method (Bauer et al, 1966) using Mueller–Hinton agar (Difco). Initially, an emulsion of sample in saline solution was prepared by adjustment to the 0.5 McFarland turbidity standards. The susceptibility of the E. coli strains was tested in relation to several antibiotics, including: chloramphenicol (CAF) (30 μg), ciprofloxacin (5 μg), gentamycin (10 μg), trimethoprim (1.25 μg-sulfamethoxazole 23.75 μg), erythromycin (15 μg), streptomycin (10 μg) and tetracycline (30 μg) (Mast Group Ltd., Merseyside, UK). Using sterile tweezers, commercially available antibiotic disks were placed individually on the surface of Mueller-Hinton agar. After 24 h of incubation at 35°C, the strains were scored as “susceptible”, “intermediate”, or “resistant” to each antibiotic based on the measurement of the inhibition zone, as recommended by clinical laboratory standard institute (CLSI).26
Trimethoprim
Manufactured by Mast Group
Sourced in United Kingdom, Germany
Trimethoprim is a synthetic antimicrobial agent commonly used in laboratory settings. It functions by inhibiting the bacterial enzyme dihydrofolate reductase, which is essential for the synthesis of tetrahydrofolate, a necessary cofactor for bacterial DNA and RNA production. This mechanism of action makes trimethoprim an effective tool for inhibiting the growth of certain bacteria in controlled laboratory environments.
Automatically generated - may contain errors
Lab products found in correlation
Ciprofloxacin, by Mast Group (2 mentions)
Streptomycin, by Mast Group (1 mentions)
Tetracycline, by Mast Group (1 mentions)
Chloramphenicol, by Mast Group (1 mentions)
Erythromycin, by Mast Group (1 mentions)
Gentamycin, by Mast Group (1 mentions)
Colistin, by bioMérieux (1 mentions)
Ceftriaxone, by Mast Group (1 mentions)
Meropenem, by Mast Group (1 mentions)
Ampicillin sulbactam, by Mast Group (1 mentions)
Amikacin, by Mast Group (1 mentions)
Ceftazidime, by Mast Group (1 mentions)
Cefepime, by Mast Group (1 mentions)
2 protocols using trimethoprim
1
Antimicrobial Susceptibility Profiling of E. coli
2
Antimicrobial Susceptibility Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Standard disc diffusion method which is recommended by Clinical Laboratory and Standard Institute (CLSI 2016) was used to evaluate antimicrobial susceptibility of the isolated organisms. Accordingly, susceptibility of the isolates to following antibiotics: amikacin (30 μg/disk), trimethoprim/sulfamethoxazole (1.25/23.75 μg/disk), cefepime (30 μg/disk), ceftriaxone (30 μg/disk), piperacilin/tazobactam (100/10 μg), ciprofloxacin (5 mg), meropenem (10 μg/disk), ceftazidime (30 μg/disk) and ampicillin-sulbactam (10/10 μg) (Mast Co., Darmstadt, Germany) were examined. In addition, Minimum Inhibitory Concentrations (MICs) were determined by the E-test method according to the manufacturer’s guidelines for colistin against A. baumannii, Klebsiella pneumoniae (K. pneumoniae), and P. aeruginosa (AB Biodisk). The MIC was read where inhibition of growth intersected the E-test strip. When small colonies grew within the zone of inhibition or a haze of growth occurred around MIC end points, the highest MIC intersect was recorded.
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