Mw 40 000

PCL nanofibers were fabricated by the use of Multispin (Nanuntio, Prague, Czech Republic). The solution for the electrospinning process contained 10% (w/v) of PCL (MW 40 000 Wako Chemicals GmbH, Neuss, Germany), dissolved in a mixture of chloroform and ethanol at a ratio of 10:1. Electrospinning was performed using a needleless wire electrode, the fibers produced were deposited on a nonwoven supporting textile (Spunbond, Pegas Textiles, Prague, Czech Republic). After fabrication, produced nanofiber towels were stored at room temperature, samples of each were collected, put on aluminum stubs and coated with a gold layer employing a Polaron sputter-coater (SC510, Polaron, Now Quorum Technologies Ltd., Lewes, UK). The samples treated with gold were studied with electron microscope Aqua SEM (Tescan, Brno-Kohoutovice, Czech Republic). To perform the experiments, nanofibers towels were sterilized, cut and the samples were soaked with the myrtle extracts from seeds, previously molecularly analyzed and described [15 (link)], and left to dry before use.

Nanofibers were prepared from polycaprolactone (PCL) polymeric solution through a needleless direct-current electrospinning method. Electrospinning was performed using 10% (w/v) PCL (MW 40,000 Wako Chemicals GmbH, Neuss, Germany) solution, wherein PCL polymeric pellets were dissolved in chloroform:ethanol at a ratio of 9:1 (v/v) on a Multispin (Nanuntio, Prague, Czech Republic) electrospinning unit, with a wire needleless electrode. The applied voltage was in a range of 57 ± 1 kV. The temperature was 24 ± 1 °C and relative humidity was 40% ± 5% during electrospinning preparation. The distance between the wire electrode and the collects was 30 cm, and the fibers produced were deposited on a non-woven supporting textile (Spunbond, Pegas Textiles, Prague, Czech Republic), as previously described [53 (link)].

Nanofibers were prepared at room temperature using the electrospinning method using a ™ NS 1S500U, Elmarco device (Elmarco, Czech Republic), with a maximal voltage of up to 100 kV. The solution for the electrospinning process contained 10% (w/v) PCL ((MW 40,000 Wako Chemicals GmbH, Neuss, Germany) dissolved in a mixture of chloroform and ethanol at a 10:1 ratio (VWR International, Radnor, PA, USA). The PCL solution used to perform the polydatin-PCL (PD-PCL) nanofiber contained a 0.5% (w/v) polydatin concentration. Electrospinning was performed using a needleless wire electrode. The fibers produced were deposited on a nonwoven supporting textile (Spunbond, Pegas Textiles, Prague, Czech Republic). After production, the nanofiber towels were stored at room temperature, samples of each towel were collected, placed on aluminum stubs, and coated with a gold layer employing a Polaron sputter-coater (SC510, Polaron, Now Quorum Technologies Ltd., Lewes, UK). The samples treated with gold were studied with an electron microscope Aqua SEM (Tescan, Brno-Kohoutovice, Czech Republic).