Chloroform d1

Chloroform-d1 (99.8%, 0.03% TMS) was obtained from Deutero GmbH (Kastellaun, Germany). FAME Mix C4-C24, Supelco PUFA-1 Marine Source, cis-6,9,12,15-octadecatetraenoic acid methyl ester (≥ 97.0%), and cis-7,10,13,16,19-docosapentaenoic acid methyl ester (≥ 98.0%) from Merck KGaA (Darmstadt, Germany) as well as cis-8,11,14,17-eicosatetraenoic acid methyl ester (98%) from Larodan AB (Solna, Sweden) were used as external standards for GC analysis.

Caproleic acid, purity ≥ 96%, oleic acid, purity ≥ 99% (GC), and α-linolenic acid, purity ≥ 99%, were purchased from Sigma-Aldrich, Chemie, GmbH, Taufkirchen, Germany. EPA, purity > 99%, and DHA, purity > 99%, were purchased from Larodan AB, Karolinska Institutet, Solna, Sweden. Chloroform-d₁ and DMSO-d₆, 99.8%, were obtained from Deutero, GmbH, Kastellaun, Germany. Molecular sieves (3Å) were obtained from Sigma-Aldrich and activation was achieved by heating at 200–230 °C for 24 h and the use of a high vacuum for 3 h.

Chemicals: chloroform-d¹, 99.6% (Deutero GmbH, Kastellaun, Germany); dichlorobenzene, anhydrous, 99% (Sigma-Aldrich Chemie GmbH, Taufkirchen, Germany), secure seal, withdrawn under counter current argon flow; dichloromethane, anhydrous, ≥99.8% (Sigma-Aldrich), secure seal, withdrawn under counter current argon flow; dioxolane, anhydrous, 99.8% (Sigma-Aldrich), secure seal, withdrawn under counter current argon flow; n-pentane, technical, >98% (Haltermann Carless, Hamburg, Germany); sulfur trioxide DMF complex, 97+ % (ACROS Organics, Geel, Belgium); triethylamine, >98% (Fluka Analytical, Merck KGaA, Darmstadt, Germany); trioxane, ≥99% (Sigma-Aldrich). These were used as supplied if not stated otherwise. Polyoxyethylene diols A, B, and C were obtained with nominal weights of 1000, 400 and 200 g/mol, respectively, as PEG 1000, (Fluka, Honeywell Specialty Chemicals Seelze GmbH, Seelze, Germany), PEG 400, (Fluka), and PEG 200 for synthesis (Merck KGaA, Darmstadt, Germany); they were then dried in an oil pump vacuum prior to used. The number average molecular weights were determined with gel permeation chromatography, being 1076, 364 and 136 g/mol, respectively.