Log In Sign up
The largest database of trusted experimental protocols

Cm10 electron microscope

Manufactured by Ametek
Visit Supplier
Sourced in Netherlands

The CM10 electron microscope is a high-performance instrument designed for advanced materials analysis. It utilizes an electron beam to generate images and data on the microstructure and composition of a wide range of samples. The CM10 provides detailed information about the surface topography, elemental distribution, and crystalline structure of the specimen under investigation.

Automatically generated - may contain errors

Lab products found in correlation

Digital camera, by Ametek (1 mentions) 1k ccd camera, by Ametek (1 mentions) Colorview iiiu camera, by Olympus (1 mentions) Tem ccd camera, by Ametek (1 mentions)

4 protocols using cm10 electron microscope

1

Ultrastructural Analysis of Spindle Pole Body

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were high-pressure frozen, freeze substituted, sectioned, and stained as previously described to examine the SPB by EM (Giddings et al., 2001 (link); Jaspersen et al., 2002 (link)). Serial thin sections were viewed on a Philips CM10 electron microscope, and images were captured with a Gatan digital camera and viewed with Digital Micrograph software.
Chen J., Gardner J.M., Yu Z., Smith S.E., McKinney S., Slaughter B.D., Unruh J.R, & Jaspersen S.L. (2019). Yeast centrosome components form a noncanonical LINC complex at the nuclear envelope insertion site. The Journal of Cell Biology, 218(5), 1478-1490.
+ Open protocol
+ Expand
2

Negative Staining of Stylo-MdtM Protein

Check if the same lab product or an alternative is used in the 5 most similar protocols
2-μL aliquots (10 – 20 μg/mL) of wild-type or mutant styMdtM were applied to glow-discharged carbon-coated copper-palladium grids and incubated for 30 seconds. Grids were washed twice with double-distilled deionized water, once with 0.75% (w/v) uranyl formate, and then stained for 30 to 40 seconds with 0.75% uranyl formate. Grids were blotted with filter paper and completely dried with vacuum suction. Grids were inspected with a Philips CM10 electron microscope operated at 100 kV and equipped with a Gatan 2K x 2K CCD camera (Model 894 US1000). Images were recorded at a defocus of –1.5 μm.
Shaheen A., Ismat F., Iqbal M., Haque A., Ul-Haq Z., Mirza O., De Zorzi R., Walz T, & Rahman M. (2021). Characterization of the multidrug efflux transporter styMdtM from Salmonella enterica serovar Typhi. Proteins, 89(9), 1193-1204.
+ Open protocol
+ Expand
3

Muscle Biopsy Analysis Workflow

Check if the same lab product or an alternative is used in the 5 most similar protocols
Open muscle biopsies were obtained from biceps brachii or quadriceps of the four patients and the healthy sibling in generation II of the family. The muscle was rapidly frozen in liquid nitrogen‐chilled isopentane and 7‐μm‐thick cryostat sections were stained for standard histological and histochemical techniques including hematoxylin and eosin, Gomori trichrome, nicotinamide adenine dinucleotide dehydrogenase (NADH), and ATPase pH 9.4. Stained sections were evaluated with an Olympus BX41 (Tokyo, Japan) equipped with a ColorView IIIu camera (Olympus).
A piece of muscle from each biopsy was fixed in glutaraldehyde, embedded in Embed 812 resin, and processed for electron microscopy by standard methods (Malfatti et al, 2013). Ultrathin sections were viewed with Philips CM10 electron microscope (Eindhoven, Netherlands) equipped with Gatan 1k CCD camera.
Servián‐Morilla E., Takeuchi H., Lee T.V., Clarimon J., Mavillard F., Area‐Gómez E., Rivas E., Nieto‐González J.L., Rivero M.C., Cabrera‐Serrano M., Gómez‐Sánchez L., Martínez‐López J.A., Estrada B., Márquez C., Morgado Y., Suárez‐Calvet X., Pita G., Bigot A., Gallardo E., Fernández‐Chacón R., Hirano M., Haltiwanger R.S., Jafar‐Nejad H, & Paradas C. (2016). A POGLUT1 mutation causes a muscular dystrophy with reduced Notch signaling and satellite cell loss. EMBO Molecular Medicine, 8(11), 1289-1309.
+ Open protocol
+ Expand
4

Ultrastructural Analysis of Frontal Cortex

Check if the same lab product or an alternative is used in the 5 most similar protocols
Frontal cortex tissues from CamKII and CamKII;(GR)go mice were cut into ~1-mm3 pieces, fixed in glutaraldehyde-containing phosphate buffer, pH 7.2, at room temperature and postfixed in 1% OsO4 solution for 1 h at 4 °C. The specimens were then dehydrated, embedded in araldite, cut into semi-thin and thin sections, stained with uranyl acetate and lead citrate, examined with a Philips CM10 electron microscope and photographed with a Gatan TEM CCD camera.
Choi S.Y., Lopez-Gonzalez R., Krishnan G., Phillips H.L., Li A.N., Seeley W.W., Yao W.D., Almeida S, & Gao F.B. (2019). C9ORF72-ALS/FTD-associated poly(GR) binds Atp5a1 and compromises mitochondrial function in vivo. Nature neuroscience, 22(6), 851-862.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!