A8511

Manufactured by Merck Group

The A8511 is a piece of laboratory equipment manufactured by Merck Group. It is designed to perform a specific core function within a laboratory setting. The detailed technical specifications and intended applications of this product are not available in this response.

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Lab products found in correlation

2 deoxy d glucose, by Merck Group (1 mentions) Propranolol, by Merck Group (1 mentions) Flexstation 3 multi mode microplate reader, by Molecular Devices (1 mentions) Tepp 46, by Merck Group (1 mentions) Flg22, by Sangon (1 mentions) P6782, by Merck Group (1 mentions)

2 protocols using a8511

Neutrophil ROS Production Measurement

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ROS production was measured by luminol-dependent chemiluminescence (CL) assay as described by Kanashiro and collaborators^{51 (link)}. Briefly, neutrophils (0.2 × 10⁶) were pre-treated (when indicated) for 1 h with oxalate (3 mM, Sigma, 71800), TEPP-46 (30 µM, Millipore, 5054870001), 2-Deoxy-D-glucose (2-DG, 3 mM, Sigma, D8375), 3PO (10 µM, Calbiochem, 525330), phosphoenolpyruvate (PEP, 1 mM, Sigma, P0564), 5-pentadecylresorcinol (5-PDR, 30 µM, Sigma, 91822) or propranolol (30 µM, Sigma, P0884). Neutrophils were then activated with Zy/op (100 µg/mL) or S. aureus (MOI = 3) in the presence of luminol (Sigma, A8511) 10⁻⁴M or lucigenin (Sigma, M8010). The reaction was monitored in a luminometer (FlexStation 3 Multi-Mode Microplate Reader, Molecular Devices, San Jose, CA) for 1 h, at 37 °C, and the results were expressed as the area under the time-course CL curve (AUC).

Toller-Kawahisa J.E., Hiroki C.H., Silva C.M., Nascimento D.C., Públio G.A., Martins T.V., Damasceno L.E., Veras F.P., Viacava P.R., Sukesada F.Y., Day E.A., Zotta A., Ryan T.A., Moreira da Silva R., Cunha T.M., Lopes N.P., Cunha F.D., O’Neill L.A, & Alves-Filho J.C. (2023). The metabolic function of pyruvate kinase M2 regulates reactive oxygen species production and microbial killing by neutrophils. Nature Communications, 14, 4280.

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Oxidative Burst Assay in Plants

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Oxidative burst assays were performed as previously described (Fabro et al., 2004) . In detail, leaf discs from 4-week-old plants were incubated in distilled water in 96-well flat-bottomed white plates overnight in the dark at room temperature (22°C). The next day, distilled water was changed to working solution containing 34 lg ml À1 luminal (A8511; Sigma-Aldrich, http://www.sigmaald-rich.com), 20 lg ml À1 peroxidase (P6782; Sigma-Aldrich) and 100 nM Flg22 (synthesized by Sangon Biotech, http://www.san-gon.com). ROS production was measured by the relative light units of luminescence using a VICTOR X5 2030 multilabel reader (Perkin Elmer, http://www.perkinelmer.com).

Wu J.X., Li J., Liu Z., Yin J., Chang Z.Y., Rong C., Wu J.L., Bi F.C, & Yao N. (2015). The Arabidopsis ceramidase AtACER functions in disease resistance and salt tolerance. The Plant journal : for cell and molecular biology, 81(5).

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