Dulbecco s modified eagle medium dmem media

Manufactured by Thermo Fisher Scientific

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Dulbecco's Modified Eagle Medium (DMEM) is a cell culture medium designed to support the growth and maintenance of a variety of cell types. It provides essential nutrients, growth factors, and other components required for cell proliferation and survival. DMEM is a widely used and well-established medium in the field of cell biology and biotechnology.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions) Bovine serum albumin (bsa), by Merck Group (2 mentions) Sodium chloride (nacl), by Merck Group (2 mentions) Calcein am, by Thermo Fisher Scientific (2 mentions) Alexa fluor 568, by Thermo Fisher Scientific (1 mentions) γh2ax antibody, by Merck Group (1 mentions) γh2ax antibody, by BD (1 mentions) A1r confocal microscope, by Nikon (1 mentions) Fortessa flow cytometer, by BD (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Mda mb 231 cell, by American Type Culture Collection (1 mentions) Diallyl disulfide, by Merck Group (1 mentions) Recombinant human tnfα, by RayBiotech (1 mentions) Hrp goat anti mouse secondary antibody, by Santa Cruz Biotechnology (1 mentions) Antibiotic antimycotic mixture, by Thermo Fisher Scientific (1 mentions) Sodium dodecyl sulfate (sds), by Merck Group (1 mentions) Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions) Sp600125, by Merck Group (1 mentions) Glycine, by Merck Group (1 mentions) Trypan blue vital stain, by Thermo Fisher Scientific (1 mentions)

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DMEM medium (4294 citations) DMEM media (655 citations) Dulbecco’s modified (295 citations)

12 protocols using dulbecco s modified eagle medium dmem media

Flow Cytometry and Immunofluorescence Analysis of A3A-Induced DNA Damage

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HEK293T cells used for flow cytometry were cultured in Dulbecco’s Modified Eagle Medium (DMEM) media (Gibco) supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin at 37°C with 5% CO₂ and cells were periodically tested to be mycoplasma negative. The cells were transfected with A3A-INS2 or A3A(E72A)-INS2, or co-transfected with A3A-SPL2_N and A3A-SPL2_C for 24 hours prior to harvest and staining with γH2AX antibody (BD Pharmigen, 647) and flow cytometry analysis. Cells were gated on FITC and APC using the Fortessa Flow Cytometer (BD Biosciences), and results were analyzed using FlowJo. The gating strategy is exemplified in Extended Fig. 8a.
U2OS cells used for immunofluorescent studies were cultured in Dulbecco’s Modified Eagle Medium (DMEM) media (Gibco) supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin at 37°C with 5% CO₂ and cells were periodically tested to be mycoplasma negative. U2OS cells plated on coverslips were transiently transfected with A3A-INS, A3A(E72A)-INS2 or co-transfected with A3A-SPL2_N and A3A-SPL2_C constructs for 24 hours prior to incubation with γH2AX antibody (Millipore Sigma) and immunofluorescent staining with Alexa Fluor 568 (Invitrogen) and DAPI. Stained cells were imaged with a Nikon A1R confocal microscope and analyzed using ImageJ.

Berríos K.N., Evitt N.H., DeWeerd R.A., Ren D., Luo M., Barka A., Wang T., Bartman C.R., Lan Y., Green A.M., Shi J, & Kohli R.M. (2021). CONTROLLABLE GENOME EDITING WITH SPLIT-ENGINEERED BASE EDITORS. Nature chemical biology, 17(12), 1262-1270.

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Antiproliferative Effects of Diallyl Disulfide on MDA-MB-231 Cells

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Cell lines, chemicals and reagents: Triple negative human breast tumor (MDA-MB-231) cells were obtained from American Type Culture Collection (Rockville, MD). Dulbecco’s Modified Eagle Medium (DMEM) media, fetal bovine serum (FBS) and penicillin/streptomycin were all obtained from Invitrogen (Carlsbad, CA). Recombinant human TNFα was purchased from RayBiotech (RayBiotech Inc., Norcross, GA, USA). Diallyl disulfide (>80%) was purchased from Sigma-Aldrich (St. Louis, MO, USA).

Bauer D., Redmon N., Mazzio E., Taka E., Reuben J., Day A., Sadrud-Din S., Flores-Rozas H., Soliman K, & Darling-Reed S. (2015). Diallyl disulfide inhibits TNFα induced CCL2 release through MAPK/ERK and NF-Kappa-B Signaling. Cytokine, 75(1), 117-126.

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Apoptosis and Cell Cycle Regulation

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UA, SP600125, MTT, Tris, glycine, NaCl, SDS, BSA, β-actin antibodies were purchased from Sigma-Aldrich (St. Louis, MO, USA). Dulbecco's Modified Eagle Medium (DMEM) media, fetal bovine serum (FBS), 0.4% trypan blue vital stain, and antibiotic-antimycotic mixture were obtained from Invitrogen (Carlsbad, CA, USA). Antibodies against procaspase-3, cyclin D1, and c-MYC as well as goat anti-rabbit-horse radish peroxidase (HRP) conjugate and goat anti-mouse HRP secondary antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Doudican N.A., Mazumder A., Kapoor S., Sultana Z., Kumar A., Talawdekar A., Basu K., Agrawal A., Aggarwal A., Shetty K., Singh N.K., Kumar C., Tyagi A., Singh N.K., Darlybai J.C., Abbasi T, & Vali S. (2014). Predictive Simulation Approach for Designing Cancer Therapeutic Regimens with Novel Biological Mechanisms. Journal of Cancer, 5(6), 406-416.

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Cellular Calcium Signaling Analysis

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CaG, ACZ, neomycin, Pyr6, and Pyr10 were purchased from Sigma–Aldrich (St. Louis, MO, USA). Dulbecco’s Modified Eagle Medium (DMEM) media, fetal bovine serum (FBS), antibiotics (penicillin and streptomycin), glutamine, and Fura-2-acetoxymethyl ester (Fura-2-AM) were purchased from Invitrogen (Carlsbad, CA, USA). All the chemicals were of analytical grade.

Gombedza F.C., Shin S., Sadiua J., Stackhouse G.B, & Bandyopadhyay B.C. (2024). The Rise in Tubular pH during Hypercalciuria Exacerbates Calcium Stone Formation. International Journal of Molecular Sciences, 25(9), 4787.

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Establishing PTX-resistant NSCLC cell lines

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293T, H460, A549, and primary human bronchial epithelial (HBE) cells were procured from Procell (China). H460 and A549 NSCLC cells resistant to PTX were created by subjecting the parent cells to higher dosages of PTX (SP8020; Solarbio, Beijing, China). The H460, A549, and HBE was cultured at 37°C with 5% CO₂ in 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin supplemented RPMI1640 media (Invitrogen, Carlsbad, CA, USA). The 293T was cultured at 37°C with 5% CO₂ in 10% FBS and 1% penicillin–streptomycin supplemented dulbecco's modified eagle medium (DMEM) media (Invitrogen, Carlsbad, CA, USA). Additionally, media was added with 5 nM PTX (Solarbio) to keep the H460/PTX and A549/PTX cells resistant.

Xia S, & Wang C. (2023). Hsa_circ_0003220 Drives Chemoresistance of Human NSCLC Cells by Modulating miR-489-3p/IGF1. International Journal of Genomics, 2023, 8845152.

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Chondroitin Sulfate B and Poly-L-Lysine Biomaterial Synthesis

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Chondroitin sulfate B sodium salt (from porcine intestinal mucosa, ≥90%, lyophilized powder, 60 kDa; PDI = 1.94) and poly-L-lysine (PLL) (0.1 % w/v in H₂O), sodium acetate, sodium chloride, potassium chloride, sodium phosphate dibasic, paraformaldehyde, Triton, bovine serum albumin (BSA), and potassium phosphate monobasic were obtained from Sigma-Aldrich (St. Louis, MO, USA). 2D clay particles (Laponite XLG) with a thickness of 1 nm and lateral dimensions of 20 − 50 nm were obtained from BYK Additives Inc (Rochester Hills, MI, USA). Dulbecco’s phosphate-buffered saline DPBS (Gibco), Dulbecco’s modified eagle medium (DMEM) media (Gibco), Minimum Essential Medium Alpha (MEM α), ethidium homodimer-1 (EthD-1), Calcein AM, PrestoBlue assay, Alexa Fluor 594−phalloidin, and 4′,6- diamidino-2-phenylindole (DAPI), and rhodamine-phalloidin were purchased from Thermo Fisher Scientific (USA). QuantiChrom Calcium Assay kit, and QuantiChrom Alkaline Phosphatase Activity kit were purchased from BioAssay Systems (Hayward, CA, USA).

Zandi N., Sani E.S., Mostafavi E., Ibrahim D.M., Saleh B., Shokrgozar M.A., Tamjid E., Weiss P.S., Simchi A, & Annabi N. (2020). Nanoengineered Shear-Thinning and Bioprintable Hydrogel as a Versatile Platform for Biomedical Applications. Biomaterials, 267, 120476.

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ARA 290 Modulates Schwann Cell Inflammation

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Rat RSC96 Schwann cells were grown in complete Dulbecco’s modified eagle medium (DMEM) media (Gibco, Grand Island, NY) containing penicillin (100 U/mL), streptomycin (100 U/mL) and 10% fetal calf serum at 37°C and 5% CO₂.
To analyse the effects of ARA 290 on inflammatory cytokine expression by RSC96 Schwann cells, 10⁶ cells were seeded into 6-well cell culture plates in triplicates and cultured overnight. Afterwards cells were washed twice by PBS, and then ARA 290 of different doses were added into the culture with or without 1 µg/ml lipopolysaccharide (LPS) for overnight. Thereafter, mRNA levels of inflammatory cytokines were measured using following primers: TNF-α (sense, TGA TCG GTC CCA ACA AGG A; antisense, TGC TTG GTG GTT TGC TAC GA), iNOS (sense, TCT GTG CCT TTG CTC ATG ACA; antisense, TGC TTC GAA CAT CGA ACG TC) and β-actin (sense, CCG TCT TCC CCT CCA TCG T; antisense, ATC GTC CCA GTT GGT TAC AAT GC).
In Schwann cell proliferation assays, cells were cultured in 1% fetal calf serum and treated with different doses of ARA 290 for 24 h and compared to proliferation of cells in normal 10% fetal calf serum. Cell proliferation was determined by Cell Counting Kit-8 Assay (Dojindo, Kumamoto, Japan) according to the manufacturer’s protocol.

Liu Y., Luo B., Han F., Li X., Xiong J., Jiang M., Yang X., Wu Y, & Zhang Z. (2014). Erythropoietin-Derived Nonerythropoietic Peptide Ameliorates Experimental Autoimmune Neuritis by Inflammation Suppression and Tissue Protection. PLoS ONE, 9(3), e90942.

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Apoptosis Induction in MCF-7 Breast Cancer Cells

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Anastrozole (ANS) (molecular weight 293.37, purity ≥ 98% (HPLC), polyvinyl alcohol (PVA), polycaprolactone (PCL, Mw 42,000 Da), stearic acid (SA), and PEG 6000 were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). The breast cancer cell line MCF-7 (ATCC HTB-22) was purchased from the American Type Culture Collection (Manassas, VA, USA). The cells were cultured in Dulbecco’s modified Eagle medium DMEM media from Gibco Laboratories (Gaithersburg, MD, USA) containing 10% fetal bovine serum and 1% L-glutamine cells and were incubated at 37 °C in a 5% CO₂ humidified incubator. An Annexin V-FITC apoptosis detection kit (BMS500FI-100, was purchased from Invitrogen, Carlsbad, CA, USA). Cells were harvested using the appropriate amount of trypsin Triple Express 1× from (Gibco Laboratories, Gaithersburg, MD, USA). All other reagents and chemicals were of analytical grade.

Massadeh S., Omer M.E., Alterawi A., Ali R., Alanazi F.H., Almutairi F., Almotairi W., Alobaidi F.F., Alhelal K., Almutairi M.S., Almalik A., Obaidat A.A., Alaamery M, & Yassin A.E. (2020). Optimized Polyethylene Glycolylated Polymer–Lipid Hybrid Nanoparticles as a Potential Breast Cancer Treatment. Pharmaceutics, 12(7), 666.

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Cell Viability and Apoptosis Assays

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Dulbecco's modified eagle medium (DMEM) media, fetal bovine serum (FBS), and Trypsin were from Gibco-BRL (UK). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was purchased from Sigma-Aldrich (Germany). Annexin V-FITC Apoptosis Detection Kit was obtained from eBioscience (Austria).

Naderi J., Dana N., Javanmard S.H., Amooheidari A., Yahay M, & Vaseghi G. (2020). Effects of standardized Cannabis sativa extract and ionizing radiation in melanoma cells in vitro. Journal of cancer research and therapeutics, 16(6).

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Monocyte-Macrophage Differentiation and Head Neck Cancer Cell Culture

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Human THP-1 monocytes were cultured in Roswell Park Memorial Institute (RPMI) medium with 10% FBS, 1% penicillin, and streptomycin. Cells were incubated at 37°C in a 5% v/v CO₂ atmosphere. For monocyte–macrophage differentiation, cells were seeded at a density of 2.5×10⁵ cells/mL, and macrophage differentiation was induced by exposing the cells to 100 nM/mL phorbol-12-myristate-13-acetate (Invivogen) for 24 hours and replaced with fresh media for 24 hours. Head and neck cancer cell lines (Cal27, FaDu) were grown in complete Dulbecco's Modified Eagle Medium (DMEM) media (Gibco) supplemented with 10% heat-inactivated fetal bovine serum (FBS) (Corning), 1% penicillin, and streptomycin (Corning). SCC7, a murine head and neck carcinoma cell line, was cultured in DMEM media supplemented with 10% FBS, 2 mM L-glutamine, and 100 U/mL penicillin/streptomycin.

Tanagala K.K., Morin-Baxter J., Carvajal R., Cheema M., Dubey S., Nakagawa H., Yoon A., Cheng Y.S., Taylor A., Nickerson J., Mintz A, & Momen-Heravi F. (2022). SP140 inhibits STAT1 signaling, induces IFN-γ in tumor-associated macrophages, and is a predictive biomarker of immunotherapy response. Journal for Immunotherapy of Cancer, 10(12), e005088.

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