Mice were assigned to control (n = 7) or MLi-2 (n = 8) groups to equally match sex. Administration of MLi-2 or control diet began 3 days prior to α-synuclein PFF injection. Control diet (Research Diets D01060501) was the same as the MLi-2 diet (Research Diets D17031301) except for the compound and a dye to allow visual discrimination between the two diets. MLi-2 was incorporated at 240 mg/kg diet to achieve approximately 30 mg/kg/day dosing based on approximately 3–4 g diet consumption/day in mice that were approximately 25 g and stayed the same weight for the course of the study. This dose was selected based on previous work with this molecule [11 (link)]. Mice were weighed once a week and diet was weighed and replenished every 2–3 days to allow assessment of estimated dosage.
D01060501
Manufactured by Research Diets
D01060501 is a laboratory equipment product. It serves as a core function, but a detailed description cannot be provided while maintaining an unbiased and factual approach without extrapolation.
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Lab products found in correlation
4 protocols using d01060501
1
Neuroprotective Effects of MLi-2 in α-Synuclein PFF Mouse Model
2
Synthesis and Formulation of MLi-2 Diet
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MLi-2 was synthesized at Merck & Co., Inc. (Boston, MA). Diet was prepared at Research Diets (New Brunswick, NJ), and formulated to deliver a dose of 0, 10, or 60 mg/kg MLi-2 per day, based on an average daily food consumption of 4 g per 30 g mouse. The base for each diet was the vehicle diet (D01060501; Research Diets).
3
Comparison of Semi-Purified and Natural Diets
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In the present study, two diets were used, a semi-purified diet (D01060501; Research Diets, Inc.) and a ND (A04; Panlab). In the SD, the main source of protein was casein. Maize starch and maltodextrin were the main contributors to the carbohydrate content, whereas cellulose was the fibre source. In contrast, the ND was prepared from natural ingredients. Proteins were mainly provided by fish, soyabean meal and yeast, and carbohydrates and fibre came from cereals, bran and middling. Concerning the lipid composition, although both diets can be considered as normal-fat diets, they differed in fat content (3•26 % in the ND and 5•08 % in the SD) and its sources (cereals, such as maize grain and barley, fish and soyabean meal for the ND and lard and soyabean oil for the SD). To have a comparable composition between the diets, the gross composition of both diets was analysed by a reference analytical laboratory (Laboratori Dr Vidal-general Lab SL), as shown in Table 1.
4
Chemotherapy-Induced Liver Injury in Mice
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Based on the previous studies, 12, (link)13 (link) C57Bl/6 mice were treated with an intraperitoneal injection of L-OHP 6 mg/kg, followed by 5-FU 50 mg/kg and l-LV 90 mg/kg 2 h later on a weekly basis for 5 weeks. Control animals received vehicle alone. Mice were fed on a standard purified diet (D01060501; Research Diets Inc.) instead of a standard chow diet. 12, (link)13 (link) All animals received standard animal house care, including ad libitum access to water and the purified diet. Mice were killed 1 week after the final dose of chemotherapy, and blood and liver samples were obtained. Serum AST and ALT levels were evaluated in serum samples. Liver samples were evaluated by light microscopy and electron microscopy.
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