Whole-mount fluorescent in situ hybridization was performed as previously described using the fgf10 probe(16 (link)). Whole mount IF staining was performed as previously described (16 (link)). Antibodies used include rabbit anti-Prox1a (1:100, GTX128354, GeneTex), goat anti-Hnf4a (1:50, sc6556, Santa Cruz), mouse anti-Alcama (1:20, zn-8, DSHB) and mouse anti-Islet1/2 (1:20, 39.4D5, DSHB), rabbit anti-pan-Cadherin (1:5000; Sigma), mouse anti-Anxa4 (1:100, ab71286, aka 2F11, Abcam)(15 (link)), rabbit anti-Bhmt (1:200, ab96415, Abcam), mouse anti-Mdr1(1:200, sc-71557, Santa Cruz), chicken anti-GFP (1:300, GFP1010, Aves Labs), goat anti-mCherry (1:500, LS-C204207, LSBio), rabbit anti-Cytokeratin7 (1:200, ab181598, Abcam), mouse anti-Sox9 (1:200, ab76997, Abcam). Imaging was performed on a Zeiss LSM710 running Zen 2010 (Black) and images were processed with Adobe Photoshop.
Ab96415
Manufactured by Abcam
Ab96415 is a laboratory equipment product. It is a 96-well microplate designed for various biochemical and cell-based assays.
Automatically generated - may contain errors
Lab products found in correlation
Lsm 710, by Zeiss (2 mentions)
Ab76997, by Abcam (1 mentions)
Ab181598, by Abcam (1 mentions)
Gtx128354, by GeneTex (1 mentions)
Rabbit anti pan cadherin, by Merck Group (1 mentions)
Sc 71557, by Santa Cruz Biotechnology (1 mentions)
Sc 6556, by Santa Cruz Biotechnology (1 mentions)
Gfp 1010, by Aves Labs (1 mentions)
Ab180772, by Abcam (1 mentions)
Ab8245, by Merck Group (1 mentions)
Ab28947, by Abcam (1 mentions)
P0217, by Agilent Technologies (1 mentions)
Imagequant las 4000 system, by GE Healthcare (1 mentions)
A1978, by Merck Group (1 mentions)
P0161, by Agilent Technologies (1 mentions)
Protease inhibitor, by Roche (1 mentions)
2 protocols using ab96415
1
Multimarker Fluorescent Imaging of Liver
2
Comprehensive Protein Expression Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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ES cells were lysed in RIPA buffer supplemented with protease inhibitors (11836170001; Roche). Twelve percent SDS-PAGE gels were used for protein separation followed by transfer onto PVDF membrane. Blots were blocked with 5% non-fat milk at room temperature for 1 h and incubated with the following primary antibodies overnight at 4 °C: RNF126 (Abcam, ab234812, 1:500), BHMT (Abcam, ab96415, 1:500), NQO1 (Abcam, ab28947, 1:500), KRAS (Abcam, Ab180772, 1:500), S100A6 (Abcam, ab134149, 1:1000), OCT4 (Millipore, MABD76, 1:1000), and ESRRB (Perseus proteomics, PP-H6705-00, 1:1000). Primary antibodies for GAPDH (Abcam, Ab8245, 1:1000) and ACTB (Sigma, A1978, 1:1000) were employed as internal controls and for 1 h at room temperature. HRP-swine-anti-rabbit (Dako, P0217) and HRP-rabbit anti mouse (Dako, P0161) were used as secondary antibodies and subsequently signal was detected using ECL kit (Pierce, 32106) and the ImageQuant LAS 4000 system (GE Healthcare Life Sciences). Western blots were repeated for two times.
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