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Opteia mouse tnf elisa kit

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The BD OptEIA™ Mouse TNF ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) used for the measurement of mouse tumor necrosis factor (TNF) in cell culture supernatants, serum, and plasma.

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Lab products found in correlation

Opteia mouse il 6 elisa kit, by BD (3 mentions) Mouse il 1 beta il 1f2 duoset elisa, by R&D Systems (1 mentions) Rneasy plus mini kit, by Qiagen (1 mentions) Ldh cytotoxicity assay kit, by Beyotime (1 mentions) Quantikine elisa kit, by R&D Systems (1 mentions) Cobas c311, by Roche (1 mentions) Il 18 mouse elisa kit, by Thermo Fisher Scientific (1 mentions) Propidium iodide, by Thermo Fisher Scientific (1 mentions) Granulocyte macrophage colony stimulating factor gm csf, by Immunotools (1 mentions) Collagen, by Merck Group (1 mentions) Onecomp beads, by Thermo Fisher Scientific (1 mentions) Mowiol, by Merck Group (1 mentions) Hoechst, by Thermo Fisher Scientific (1 mentions) Alexa fluor 647, by Thermo Fisher Scientific (1 mentions) Rnase, by Thermo Fisher Scientific (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions) Hoechst 33342, by Merck Group (1 mentions) Penicillin streptomycin, by Lonza (1 mentions) Fetal bovine serum (fbs), by Lonza (1 mentions) Dulbecco s modified eagle medium dmem, by Lonza (1 mentions)

4 protocols using opteia mouse tnf elisa kit

1

Quantifying Inflammation Markers in Cell Infection

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After infection for 6, 24 and 48 h, cell culture media were collected for quantitative determination of the lactate dehydrogenase (LDH) activities by LDH Cytotoxicity Assay Kit (Beyotime, Haimen, China) and the proinflammatory cytokines IL-6, TNF-α and IL-1β by employing BD OptEIA™ Mouse IL-6 ELISA Kit (BD), BD OptEIA™ Mouse TNF ELISA Kit (BD) and Mouse IL-1 beta/IL-1F2 DuoSet ELISA (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s manual. Total mRNA from infected mice tissues was extracted using the RNeasy Plus Mini kit (QIAGEN, Hilden, Germany), followed by reverse transcription. mRNA levels of proinflammatory cytokines IL-6, TNF-α and IL-1β were determined by RT-PCR using primers listed in Table 1.
Xia A., Li X., Quan J., Chen X., Xu Z, & Jiao X. (2021). Mycobacterium tuberculosis Rv0927c Inhibits NF-κB Pathway by Downregulating the Phosphorylation Level of IκBα and Enhances Mycobacterial Survival. Frontiers in Immunology, 12, 721370.
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2

Quantification of Liver Markers and Lipids

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Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and triglyceride (TG) levels were determined using commercial kits on a Cobas c311 autoanalyzer (Roche Diagnostics, Germany). The serum levels of monocyte chemoattractant protein1 (MCP1) were measured using a commercial Quantikine ELISA Kit supplied by R&D Systems, Inc., USA. Total liver lipids were extracted, and hepatic TG and total cholesterol (TC) content was quantified using triglyceride and total cholesterol test kits from Agappe Diagnostics, India. The liver TNFα, malondialdehyde (MDA) and collagen content levels were measured in tissue homogenates using BD OptEIA Mouse TNF ELISA kit (BD Biosciences, USA), QuantiChrom TBARS Assay Kit (BioAssays Systems Inc, USA) and total collagen assay kit (QuickZyme Biosciences, The Netherlands) respectively; more details are described in Data S1.
Jain M.R., Giri S.R., Bhoi B., Trivedi C., Rath A., Rathod R., Ranvir R., Kadam S., Patel H., Swain P., Roy S.S., Das N., Karmakar E., Wahli W, & Patel P.R. (2017). Dual PPARα/γ agonist saroglitazar improves liver histopathology and biochemistry in experimental NASH models. Liver International, 38(6), 1084-1094.
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3

Cytokine Quantification by ELISA

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Human IL-1b and IL-18 concentrations were determined by ELISA using BD OptEIA Human IL-1b ELISA Kit II (BD Biosciences, San Jose, Calif) and IL-18 Human Instant ELISA Kit (Invitrogen), respectively. Mouse IL-1b, IL-18, IL-6, and TNF-a were measured by BD OptEIA Mouse IL-1b ELISA Kit (BD Biosciences), IL-18 Mouse ELISA Kit (Invitrogen), BD OptEIA Mouse IL-6 ELISA Kit (BD Biosciences), and BD OptEIA Mouse TNF ELISA Kit (BD Biosciences).
Zhou L., Liu T., Huang B., Luo M., Chen Z., Zhao Z., Wang J., Leung D., Yang X., Chan K.W., Liu Y., Xiong L., Chen P., Wang H., Ye L., Liang H., Masters S.L., Lew A.M., Gong S., Bai F., Yang J., Pui-Wah Lee P., Yang W., Zhang Y., Lau Y.L., Geng L., Zhang Y, & Cui J. (2021). Excessive deubiquitination of NLRP3-R779C variant contributes to very-early-onset inflammatory bowel disease development. The Journal of allergy and clinical immunology, 147(1).
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4

Investigating Cellular Responses Comprehensively

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Dulbecco's Modified Eagle Medium (DMEM), penicillin/streptomycin and fetal bovine serum (FBS) were purchased from Lonza (Verviers, Belgium). FITC-dextran (42 kDa), Hoechst 33342, LPS and collagen were from Sigma-Aldrich (St. Louis, MO, USA). OneComp beads were from eBioscience. BD OptEIA mouse TNF ELISA kit, BD OptEIA mouse IL-6 ELISA kit and Human inflammatory bead assay CBA was from BD Biosciences (San Diego, CA, USA). Interleukin 4 (IL-4), Interleukin 10 (IL-10), interferon-γ (INFγ) and granulocyte macrophage colonystimulating factor (GM-CSF) were from ImmunoTools (Germany). Millecell EZ slides and Mowiol were from Millipore (Hayward, CA, USA) and Upcell plates were purchased from Nunc (Rochester, NY, USA). H 2 DCFDA-CM, DHE, N-acetyl-L-cysteine (NAC), propidium iodide, Hoechst and RNase were from, Life technologies (Grand Island, NY, USA). and Rat IgG2aκ Iso Control APC were from eBioscience (San Diego, CA, USA). γH2AX and LC3B were from cell signaling (Beverly, MA, USA) and anti-rabbit Alexa Fluor 647 were from molecular probes (Life Technology, Grand Island, NY, USA).
Solhaug A., Wisbech C., Christoffersen T.E., Hult L.O., Lea T., Eriksen G.S, & Holme J.A. (2015). The mycotoxin alternariol induces DNA damage and modify macrophage phenotype and inflammatory responses. Toxicology letters, 239(1).
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