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Rgdsp and rgesp peptides

Manufactured by Bachem

RGDSP and RGESP are synthetic peptides produced by Bachem. These peptides are widely used in research applications as tools for studying cell adhesion and signaling. RGDSP and RGESP have defined amino acid sequences and purity levels suitable for various experimental protocols.

Automatically generated - may contain errors

2 protocols using rgdsp and rgesp peptides

1

Recombinant CCN1 Proteins Production

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Recombinant CCN1 and mutant proteins (D125A, DM, CCN1-I.II, CCN1-ΔCT, and D125A-ΔCT) were produced using a baculovirus expression system in insect cells and purified by ion-exchange or immuno-affinity chromatography30 (link), 32 (link). Rabbit polyclonal antibodies against CCN1 were raised against the CCN1 vWC domain67 (link). Rabbit and mouse IgGs, echstatin, and lipopolysacharide (LPS) were from Sigma. RGDSP and RGESP peptides were from Bachem. Function-blocking mAbs against mouse integrin β1 (KM16), αv (RMV-7), β3 (2C9-G3), αvβ3 (23-C6) and β5 (KN52) were from eBioscience. Recombinant murine interleukin-4 (IL-4) were from R&D systems. Horse radish peroxidase (HRP)-conjugated anti-mouse and anti-rabbit secondary antibodies were from Amersham Biosciences. Biotin-conjugated goat anti-rabbit IgG, biotin-conjugated goat anti-rat IgG antibody, and streptavidin-conjugated HRP were from Invitrogen.
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2

Recombinant CCN1 Proteins Production

Check if the same lab product or an alternative is used in the 5 most similar protocols
Recombinant CCN1 and mutant proteins (D125A, DM, CCN1-I.II, CCN1-ΔCT, and D125A-ΔCT) were produced using a baculovirus expression system in insect cells and purified by ion-exchange or immuno-affinity chromatography30 (link), 32 (link). Rabbit polyclonal antibodies against CCN1 were raised against the CCN1 vWC domain67 (link). Rabbit and mouse IgGs, echstatin, and lipopolysacharide (LPS) were from Sigma. RGDSP and RGESP peptides were from Bachem. Function-blocking mAbs against mouse integrin β1 (KM16), αv (RMV-7), β3 (2C9-G3), αvβ3 (23-C6) and β5 (KN52) were from eBioscience. Recombinant murine interleukin-4 (IL-4) were from R&D systems. Horse radish peroxidase (HRP)-conjugated anti-mouse and anti-rabbit secondary antibodies were from Amersham Biosciences. Biotin-conjugated goat anti-rabbit IgG, biotin-conjugated goat anti-rat IgG antibody, and streptavidin-conjugated HRP were from Invitrogen.
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