β2 spectrin

Manufactured by Merck Group

β2 spectrin is a structural protein found in the cytoskeleton of cells. It plays a key role in maintaining the structural integrity and organization of the cell membrane.

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Lab products found in correlation

Gabra1, by Abcam (1 mentions) β actin, by Merck Group (1 mentions) Nmiib, by BioLegend (1 mentions) A1 confocal microscope, by Nikon (1 mentions)

2 protocols using β2 spectrin

Antibody Characterization for Cell Biology

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The following antibodies were used for immunoprecipitation (IP), immunoblot (IB) or immunocytochemistry (ICC): Gabra1 (IP, IB – 1:1000, mouse, Antibodies Inc 75–136), myc (IP, mouse, Thermo Scientific PIER88843), GFP (IB – 1:1000, rabbit, Cell Signaling 2956), GFP (ICC – 1:1000, chicken, Abcam ab13970), AnkG (ICC – 1:1000, rabbit, SYSY 386003), VGAT (IB - 1:1000, ICC - 1:1000, guinea pig, SYSY 131004), β2 spectrin (IB – 1:1000, ICC0 - 1:500, mouse, Sigma-Aldrich SAB4200662), β4 spectrin (IB – 1:1000, ICC – 1:500, mouse, Antibodies Inc 75–377), Gabra1 (ICC – 1:1000, rabbit, Abcam ab33299), β-actin (IB - 1:5000, mouse, Sigma-Aldrich A1978), α2 spectrin (IB – 1:1000, rabbit, Cell Signaling 2122), Gephyrin (IB – 1:1000, rabbit, Cell Signaling 14304).

Smalley J.L., Cho N., Ng S.F., Choi C., Lemons A.H., Chaudry S., Bope C.E., Dengler J.S., Zhang C., Rasband M.N., Davies P.A, & Moss S.J. (2023). Spectrin-beta 2 facilitates the selective accumulation of GABAA receptors at somatodendritic synapses. Communications Biology, 6, 11.

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Immunolabeling of Cytoskeletal Elements

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Immunolabeling of cytoskeletal elements was performed in isolated basilar papillae, fixed in 4% paraformaldehyde for 30 min at room temperature, blocked in 10% goat serum for 1 h, and labeled with primary antibodies to α2-spectrin (monoclonal; Thermo Fisher, Waltham, MA), β2-spectrin (monoclonal; Sigma-Aldrich), and the nonmuscle myosin NMIIB (MYH10 gene, polyclonal; Biolegend) all at 1:100 dilution for 2 h at room temperature. Preparations were then labeled with secondary antibodies (rabbit or mouse conjugated with Alexa-488 or Alexa-568, 1:300, 1.5 h) and with Alexa-647 phalloidin, mounted using Fluoromount G and viewed on a Nikon A1 confocal microscope with ×100, 1.4 numerical aperture objective.

Beurg M., Gamble T., Griffing A.H, & Fettiplace R. (2022). Atypical tuning and amplification mechanisms in gecko auditory hair cells. Proceedings of the National Academy of Sciences of the United States of America, 119(12), e2122501119.

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