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2 protocols using sodium nitrite nano2

1

Analytical Reagents for Food Research

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The chemicals used in the study were obtained from the suppliers described below: choline chloride (ChChl) (≥98%), D-(-)-fructose (Fruc) (≥99%), α-D-glucose (Glu) (96%), D-(+)-xylose (Xy) (≥99.0%), DL-lactic acid (LA) (90%), Red Nile, and Folin–Ciocalteau reagent were purchased from Sigma-Aldrich (Steinheim, Germany). Glycerol (Gly) was from Glentham Life Science (Corsham, UK). DL-malic acid (MA) (≥98%) was from Thermo Fisher (Kendel, Germany). L-(+)-tartaric acid (TA) (>99.0%) was purchased from TCI (Zwijndrecht, Belgium). Citric acid (CA) (≥99.9%), anhydrous sodium carbonate (Na2CO3), and sodium nitrite (NaNO2) were purchased from VWR Chemicals (Leuven, Belgium). L-proline (LP) was from Guinama (Valencia, Spain). Betaine (Bet) was acquired from Fluorochem (Hadfield, UK), and aluminum chloride (AlCl3.6H2O) was purchased from Acofarma (Terrassa, Barcelona). Absolute ethanol was purchased from J.T Baker Chemical Co. (Deventer, The Netherlands).
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2

Phytochemical Analysis of Zinkuran SC

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Zinkuran SC was acquired from Arysta LifeScience Slovakia s.r.o. (Nové Zámky, Slovakia). Sodium selenate was purchased from Alfa Aesar (Karlsruhe, Germany). Nitric acid (HNO3, for trace element analysis) was obtained from LGC Standards (Molsheim, France), and hydrogen peroxide (H2O2) 30% (Suprapur) was obtained from Merck/VWR (Leuven, Belgium). Methanol was purchased from Biosolve (Valkenswaard, the Netherlands). Acetone, sodium carbonate (Na2CO3), and sodium hydroxide (NaOH) were acquired from Merck (Darmstadt, Germany). Ascorbic acid (vitamin C), (+)-catechin, gallic acid, Folin–Ciocalteu's phenol reagent, 2,4,6-tri(2-pyridyl)-s-triazine (TPTZ), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) diammonium salt, 2–2′-azobis (2-methylpropionamidine) dichloride (AAPH), aluminum chloride (AlCl3), and iron(II) sulfate were obtained from Sigma–Aldrich (St. Louis, MO, USA). Hydrogen chloride (HCl) and sodium nitrite (NaNO2) were obtained from VWR (Leuven, Belgium). Iron(III) chloride 6-hydrate was purchased from Merck (Darmstadt, Germany).
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