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Anti keratin 6

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Anti-Keratin 6 is a monoclonal antibody that recognizes keratin 6, a type II cytokeratin expressed in stratified and specialized epithelial cells. It is commonly used in immunohistochemistry and immunocytochemistry applications to detect and localize keratin 6 in tissue samples and cell cultures.

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Lab products found in correlation

Dm5500b microscope, by Leica (2 mentions) Meta 710, by PerkinElmer (2 mentions) Anti keratin 10, by BioLegend (2 mentions) Anti keratin 14, by Thermo Fisher Scientific (2 mentions) Scn400 slide scanner, by Leica (2 mentions)

2 protocols using anti keratin 6

1

Multicolor Immunohistochemistry of Murine Skin

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Skin tissues were embedded in paraffin or snap frozen in OCT compound. Antigen retrieval for paraffin sections was performed in citrate buffer, pH6 for the skin sections. Anti-F4/80 (clone A3-1, MCA497G, BIO-RAD), anti-Keratin 14 (MA5-11599, Invitrogen), anti-Keratin 6 (905701, Biolegend), anti-Keratin 10 (905401, Biolegend) were used for the staining. Alexa-488, Alexa-594 and Alexa-633 fluorescence conjugated secondary antibodies were used for detection. F4/80 staining was performed on cryo sections. All sections were counterstained with DAPI. Quantification of epidermal thickness was performed by measurement of epidermal thickness in five optical fields per section. In each field, four measurements were performed. Percentage of inflamed area was determined as the percentage of inflamed versus total number of optical fields at 20x on individual skin sections. Assessment of tissue pathology was performed in a blinded fashion. All images were acquired using either a Zeiss Meta 710 confocal or PerkinElmer Spinning Disc confocal microscope for fluorescent images and brightfield images were acquired using a Leica SCN400 slide scanner or a Leica DM5500 B microscope.
Jiao H., Wachsmuth L., Kumari S., Schwarzer R., Lin J., Eren R.O., Fisher A., Lane R., Young G.R., Kassiotis G., Kaiser W.J, & Pasparakis M. (2020). Z-nucleic acid sensing triggers ZBP1-dependent necroptosis and inflammation. Nature, 580(7803), 391-395.
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2

Multicolor Immunohistochemistry of Murine Skin

Check if the same lab product or an alternative is used in the 5 most similar protocols
Skin tissues were embedded in paraffin or snap frozen in OCT compound. Antigen retrieval for paraffin sections was performed in citrate buffer, pH6 for the skin sections. Anti-F4/80 (clone A3-1, MCA497G, BIO-RAD), anti-Keratin 14 (MA5-11599, Invitrogen), anti-Keratin 6 (905701, Biolegend), anti-Keratin 10 (905401, Biolegend) were used for the staining. Alexa-488, Alexa-594 and Alexa-633 fluorescence conjugated secondary antibodies were used for detection. F4/80 staining was performed on cryo sections. All sections were counterstained with DAPI. Quantification of epidermal thickness was performed by measurement of epidermal thickness in five optical fields per section. In each field, four measurements were performed. Percentage of inflamed area was determined as the percentage of inflamed versus total number of optical fields at 20x on individual skin sections. Assessment of tissue pathology was performed in a blinded fashion. All images were acquired using either a Zeiss Meta 710 confocal or PerkinElmer Spinning Disc confocal microscope for fluorescent images and brightfield images were acquired using a Leica SCN400 slide scanner or a Leica DM5500 B microscope.
Jiao H., Wachsmuth L., Kumari S., Schwarzer R., Lin J., Eren R.O., Fisher A., Lane R., Young G.R., Kassiotis G., Kaiser W.J, & Pasparakis M. (2020). Z-nucleic acid sensing triggers ZBP1-dependent necroptosis and inflammation. Nature, 580(7803), 391-395.
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