For pigment analyses, leaf samples were frozen in liquid N2 and either used directly for pigment extraction or stored at –80 °C for up to 2 weeks until further use. Pigments were extracted by grinding frozen leaf material in a mortar after addition of 1 ml of 100% acetone. After a short centrifugation, the supernatant was filtered through a 0.2 μm membrane filter (GE Healthcare, Little Chalfont, Buckinghamshire, UK) and then subjected to HPLC analysis. Separation and quantification of pigments were done by reversed-phase chromatography as described in Färber et al. (1997) (link).
0.2 μm membrane filter
Manufactured by GE Healthcare
Sourced in United Kingdom
The 0.2 μm membrane filter is a lab equipment product designed for filtration purposes. It has a pore size of 0.2 micrometers, which allows it to effectively remove small particles and microorganisms from liquids.
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Lab products found in correlation
2 protocols using 0.2 μm membrane filter
1
Leaf Pigment Extraction and HPLC Analysis
2
Pigment Extraction and HPLC Analysis
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Intact leaves or leaf discs were harvested and immediately shock frozen in liquid N2. After pestling, pigments were extracted with 1 ml of 100% acetone. After short centrifugation, samples were filtered through a 0.2 μm membrane filter (GE Healthcare, Buckinghamshire, UK) and stored at −20°C until analysis. Pigments were separated and quantified by HPLC analysis as described (Färber et al., 1997 (link)).
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