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Hi 8014

Manufactured by Hanna Instruments
Sourced in Romania

The HI 8014 is a portable pH/mV/temperature meter. It features a large LCD display, automatic temperature compensation, and GLP (Good Laboratory Practices) compliance. The meter can measure pH, mV, and temperature, and is designed for use in a variety of laboratory and field applications.

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4 protocols using hi 8014

1

In-situ pH Measurement Protocol

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The pH of samples was taken in-situ using the HANNA instrument HI 8014. The sensor was held in the sample until the pH value was stabilized within a one decimal range. Between the readings, the sensor was cleaned or rinsed in distilled water. The pH meter was calibrated before use using buffer solution of pH 4 and 9.
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2

Synthesis and Characterization of Novel Organic Compound

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All reagents were purchased from Sigma-Aldrich and used without further purification. 1H NMR and 13C NMR were measured on a VNMRJ 600 nuclear magnetic resonance spectrometer (Varian Inc., Palo Alto, CA, USA). The mass analysis was conducted with Thermo Q-Exactive Orbitrap device (Thermo Fisher Scientific Inc., Waltham, MA, USA). The melting point was determined by using the Electrothermal Melting Point Apparatus 9200. UV-vis absorption and fluorescence emission spectra were obtained using a Shimadzu 1900i spectrophotometer and Varian Cary Eclipse fluorescence spectrophotometer (Varian Inc.), respectively. Quantum yield measurements were conducted with Hamamatsu Quantaurus-QY Absolute PL quantum yield spectrometer. The pH values were adjusted by using the HI-8014 (HANNA) pH meter.
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3

Synthesis and Characterization of Novel Organic Compound

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All reagents were purchased from Sigma-Aldrich and used without further purification. 1H NMR and 13C NMR were measured on a VNMRJ 600 nuclear magnetic resonance spectrometer (Varian Inc., Palo Alto, CA, USA). The mass analysis was conducted with Thermo Q-Exactive Orbitrap device (Thermo Fisher Scientific Inc., Waltham, MA, USA). The melting point was determined by using the Electrothermal Melting Point Apparatus 9200. UV-vis absorption and fluorescence emission spectra were obtained using a Shimadzu 1900i spectrophotometer and Varian Cary Eclipse fluorescence spectrophotometer (Varian Inc.), respectively. Quantum yield measurements were conducted with Hamamatsu Quantaurus-QY Absolute PL quantum yield spectrometer. The pH values were adjusted by using the HI-8014 (HANNA) pH meter.
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4

Oxidative Stress Assay Protocol

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We measured the oxidative redox potential (ORP) values (HI 8014, portable pH/ORP meter, HANNA Instruments, Cluj-Napoca, Romania) and pH values (Denver Instrument UltraBasic UB-10 pH Benchtop meter, Cole-Parmer, Vernon Hills, Illinois, USA) of the samples. We determined the scavenging H2O2 and HOCl activity of the samples using a modified luminol-ultrasensitive chemiluminescence assay [21] (link).
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