Laser confocal microscope (VK3050, Keyence) was used to obtain surface roughness measurements using the laser source (Red 661 nm), and focus variation method for 3D profiles, and 2D features measurements of the channels and ports on the microfluidic cartridges.
Effects of the Bis[3 (trimethoxysilyl)propyl]amine, (technical grade, ≥90% Bis-TPA 413356-50ML, Sigma Aldrich) reagent on the microfluidic chips was characterized by UV-Visible spectroscopy, and Contact Angle Measurements (CAM). UV-Visible spectroscopy was performed using an Evolution 220 (Thermo Scientific) from 200 to 1000 nm spectra range on clear PMMA, PC substrates and using glass substrates as references for each corresponding set. Both different concentrations of the Bis-TPA treatments for surface functionalization (i.e., 0.1, 1, and 2% V/V with Ethanol), and the method of overnight drying (ambient and oven) were evaluated. Lastly, CAM was obtained using a Dataphysics automatic goniometer (DataPhysics Instruments USA Corp) dispensing 2 μL and 3 μL water drops onto control, plasma treated (O2 Plasma@40 cc/min for 100 seconds), and Bis-TPA functionalized (0.1%, 1%, and 2% V/V concentration with Ethanol) samples. Both, standard Right-Left (RL) angle and Young-Laplace (YL) measurements were evaluated.
Effects of the Bis[3 (trimethoxysilyl)propyl]amine, (technical grade, ≥90% Bis-TPA 413356-50ML, Sigma Aldrich) reagent on the microfluidic chips was characterized by UV-Visible spectroscopy, and Contact Angle Measurements (CAM). UV-Visible spectroscopy was performed using an Evolution 220 (Thermo Scientific) from 200 to 1000 nm spectra range on clear PMMA, PC substrates and using glass substrates as references for each corresponding set. Both different concentrations of the Bis-TPA treatments for surface functionalization (i.e., 0.1, 1, and 2% V/V with Ethanol), and the method of overnight drying (ambient and oven) were evaluated. Lastly, CAM was obtained using a Dataphysics automatic goniometer (DataPhysics Instruments USA Corp) dispensing 2 μL and 3 μL water drops onto control, plasma treated (O2 Plasma@40 cc/min for 100 seconds), and Bis-TPA functionalized (0.1%, 1%, and 2% V/V concentration with Ethanol) samples. Both, standard Right-Left (RL) angle and Young-Laplace (YL) measurements were evaluated.