Gatan cryo holder model 626

Manufactured by Ametek

Sourced in United States

The Gatan cryo-holder (model 626) is a specialized piece of lab equipment designed for cryogenic sample preparation and observation. It is used to maintain specimens at low temperatures during electron microscopy analysis.

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Lab products found in correlation

Jem 2010f, by Ametek (2 mentions) Vitrobot 3, by Thermo Fisher Scientific (2 mentions) Jem 2010f, by JEOL (2 mentions)

Spelling variants of this product

626 cryo-holder (42 citations) Gatan 626 (40 citations) Gatan 626 cryo-holder (36 citations) Cryo-holder (26 citations) Model 626 (9 citations) Gatan model 626 (3 citations) Cryo-holder model 626 (2 citations) Gatan Cryo Transfer Holder model 626.6 (2 citations)

2 protocols using gatan cryo holder model 626

Cryo-TEM Imaging of Frozen Hydrated Samples

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Quantifoil copper 200 mesh R 3.5/1 grids were washed overnight with acetone. To prepare a frozen, hydrated grid, 2.5 μL of sample was applied to the grid, blotted, and plunged into liquid ethane using Vitrobot III (FEI, Hillsboro, OR, USA). Images were collected at liquid nitrogen temperature (∼100 K) on a JEM-2010F (JEOL, Tokyo, Japan) cryo-TEM equipped with a field emission gun. JEM-2010F was operating at 200 kV and was equipped with a Gatan cryo-holder (model 626) (Gatan, Pleasanton, CA, USA). Images were recorded on DE-12, a 12.6-megapixel (3,072 × 4,096) direct detection device sensor (Direct Electron, San Diego, CA, USA). Samples were imaged at ×13,900 effective magnification targeted at 3–4 μm under focus. We used a total specimen exposure for each image of 30 e⁻/Å² over 1 s.

Kim T., Viard M., Afonin K.A., Gupta K., Popov M., Salotti J., Johnson P.F., Linder C., Heldman E, & Shapiro B.A. (2020). Characterization of Cationic Bolaamphiphile Vesicles for siRNA Delivery into Tumors and Brain. Molecular Therapy. Nucleic Acids, 20, 359-372.

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Cryo-EM Imaging of Bolas and RNA

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Quantifoil Copper 200 mesh R 3.5/1 grids were washed overnight with acetone. To prepare a frozen, hydrated grid, 2.5 μL of sample of bolas alone or bola/DS RNA (designed against GFP) was applied to the grid, blotted, and plunged into liquid ethane using Vitrobot III (FEI, Hillsboro, OR). Images were collected at liquid nitrogen temperature (∼100 K) on a JEM-2010F (JEOL, Tokyo, Japan) transmission electron cryo-microscope equipped with a field emission gun. JEM-2010F was operating at 200 kV and was equipped with a Gatancryo-holder (model 626) (Gatan, Pleasanton, CA). Images were recorded on DE-12, a 12.6 megapixel (3,072 × 4,096) Direct Detection Device sensor (Direct Electron LP, San Diego, CA). Samples were imaged at 13,900X effective magnifications targeted at 3–4 μm under focus. We used a total specimen exposure for each image of 30 e-/Å² second.

Gupta K., Afonin K.A., Viard M., Herrero V., Kasprzak W., Kagiampakis I., Kim T., Koyfman A.Y., Puri A., Stepler M., Sappe A., KewalRamani V.N., Grinberg S., Linder C., Heldman E., Blumenthal R, & Shapiro B.A. (2015). Bolaamphiphiles as Carriers for siRNA Delivery: From Chemical Syntheses to Practical Applications. Journal of controlled release : official journal of the Controlled Release Society, 213, 142-151.

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