Tetraisopropyl pyrophosphoramide (iso-OMPA) was purchased from Sigma-Aldrich and prepared in ethanol. BW284c51, ethopropazine, 2-PAM (pyridine-2-aldoxime), sodium fluoride (NaF) and donepezil were purchased from Sigma-Aldrich and prepared in deionized water. Edrophonium was purchased from Santa Cruz Biotechnology (Dallas, TX) and stocks were prepared in water. Tacrine hydrochloride (Spectrum Chemical, New Brunswick, NJ) stocks were prepared in phosphate buffer. RS194B was synthesized and purified as previously described (Radić et al. 2012 (link)). The OPs (chlorpyrifos oxon, diazinon oxon, dichlorvos, malaoxon, and paraoxon) and carbamylating agents (carbaryl, physostigmine (eserine), pyridostigmine, and neostigmine) were purchased from Sigma-Aldrich with the exception of chlorpyrifos oxon and diazinon oxon, which were purchased from Chem Service (West Chester, PA). Stocks were prepared in ethanol and further diluted in water or buffer, with the exception of physostigmine which was prepared in dimethyl sulfoxide (DMSO, Sigma-Aldrich). Solvent (ethanol or DMSO) content in inhibition or reactivation reactions was never above 1% in the samples and controls.
Malaoxon
Manufactured by Merck Group
Sourced in United States
Malaoxon is a laboratory reagent used for analytical purposes. It is a metabolite of the insecticide malathion. Malaoxon can be utilized in various analytical techniques, such as chromatography, to detect and quantify the presence of malathion in environmental or biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Paraoxon, by Merck Group (2 mentions)
Neostigmine, by Merck Group (1 mentions)
Pyridostigmine, by Merck Group (1 mentions)
Dichlorvos, by Merck Group (1 mentions)
Carbaryl, by Merck Group (1 mentions)
Ethopropazine, by Merck Group (1 mentions)
Physostigmine, by Merck Group (1 mentions)
Bw284c51, by Merck Group (1 mentions)
Tetraisopropyl pyrophosphoramide iso ompa, by Merck Group (1 mentions)
Physostigmine eserine, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Donepezil, by Merck Group (1 mentions)
Chlorpyrifos oxon, by Chem Service (1 mentions)
Eserine, by Merck Group (1 mentions)
2 tridecanone, by Merck Group (1 mentions)
α naphthyl acetate, by Merck Group (1 mentions)
β naphthol, by Merck Group (1 mentions)
Fast blue b salt, by Merck Group (1 mentions)
Fenthion, by Merck Group (1 mentions)
Atrazine, by Merck Group (1 mentions)
4 protocols using malaoxon
1
Inhibition and Reactivation of Cholinesterases
2
Biochemical Analysis of Hydrolytic Enzymes
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2-tridecanone, Fast Blue B salt [o-Dianisidine bis(diazotized) zinc double salt; DBB], sodium dodecyl sulfate (SDS), α-naphthyl acetate (α-NA), β-naphthyl acetate (β-NA) α-naphthol, β-naphthol, malaoxon and eserine were purchased from Sigma Aldrich (St Louis, MO, USA). Stock solutions of both α-NA and β-NA were prepared in absolute ethanol.
3
Comprehensive Analysis of Pesticide Residues
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CRM for organochlorine pesticides comprised a mixture of 20 compounds viz. α-BHC, β-BHC, γ-BHC, Δ-BHC, heptachlor, Aldrin, heptachlor epoxide, trans-chlordane, cis-chlordane, endosulfan-I, endosulfan-II, endosulfan sulfate, 4,4′-DDE, 4,4′-DDD, 4,4′-DDT, dieldrin, endrin, endrin aldehyde, endrin ketone and methoxychlor; a herbicide mix of 5 compounds viz. atrazine, simazine, alachlor, metolachlor and butachlor and were procured from Sigma Aldrich (USA).
Individual standards for hexachlorobenzene, and several organophosphate pesticides including ethion, disulfoton, malaoxon, fenthion, phosalone, methyl parathion, methyl chlorpyrifos, ethyl parathion, fenitrothion, propazine and methyl paraoxon, were all procured from Sigma Aldrich (USA). Standards for malathion, acephate, phosphamidon, captafol, diazinon and monocrotophos were procured from Accustandard, Inc. (USA). A mixture of pyrethroid compounds including Cyfluthrin, L-cyhalothrin, cypermethrin-I, cypermethrin-II, cypermethrin-III, deltamethrin, pendimethalin, permethrin-I, permethrin-II, fenvalerate, and tefluthrin were also purchased from Accustandard, Inc. (USA).
Individual standards for hexachlorobenzene, and several organophosphate pesticides including ethion, disulfoton, malaoxon, fenthion, phosalone, methyl parathion, methyl chlorpyrifos, ethyl parathion, fenitrothion, propazine and methyl paraoxon, were all procured from Sigma Aldrich (USA). Standards for malathion, acephate, phosphamidon, captafol, diazinon and monocrotophos were procured from Accustandard, Inc. (USA). A mixture of pyrethroid compounds including Cyfluthrin, L-cyhalothrin, cypermethrin-I, cypermethrin-II, cypermethrin-III, deltamethrin, pendimethalin, permethrin-I, permethrin-II, fenvalerate, and tefluthrin were also purchased from Accustandard, Inc. (USA).
4
Quantification of Cholinesterase Inhibitors
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The pesticides parathion, chlorpyrifos, and malathion as well as the oxons paraoxon and malaoxon were purchased from Sigma-Aldrich GmbH (Steinheim, Germany). chlorpyrifos-oxon was purchased from LGC standards GmbH (Wesel, Germany). All standards had a purity of > 95%. AChE from electric eel (Electrophorus electricus), acetylthiocholine (ATCL), NBS, 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB), ascorbic acid, and bovine serum albumin (BSA) were purchased from Sigma-Aldrich GmbH (Steinheim, Germany). Tris(hydroxymethyl)aminomethane (TRIS) was purchased from Carl Roth GmbH + Co. KG (Karlsruhe, Germany). HCl was purchased from Merck (Darmstadt, Germany). Indoxyl acetate was purchased from Thermo Fisher Scientific (Geel, Belgium). Methanol (LC–MS grade), acetone (LC–MS grade), dichloromethane (LC–MS grade), and water (LC–MS grade) were all purchased from Th. Geyer GmbH & Co. KG (Renningen, Germany). Cyclohexane (LC–MS grade) was purchased from LGC Standards GmbH (Wesel, Germany).
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