Methyl alcohol hplc grade

CalliSpheres^® (Callisyn Biomedical-Suzhou, Inc., Suzhou, China) with sizes of 50–150, 100–300, and 300–500 μm were evaluated. Each package contains 1 g of DEB and 7 of mL normal saline, making up a total volume of about 8 mL. Idarubicin hydrochloride for injection (National Medicine Permission Number H20203345, Batch No. 200917) and iopamidol injection (National Medicine Permission Number H20203293, Batch No. 200812) were kindly provided by Chia Tai Tianqing Pharmaceutical Group Co. Ltd., Nanjing, China. Each IDA package contains hydrochloride powder with 10 mg of active pharmaceutical ingredients (APIs). Idarubicin hydrochloride standard (>99%) was purchased from the National Institutes for Food and Drug Control, China. Methyl alcohol (HPLC grade) was purchased from Sigma-Aldrich, Saint Louis, MO, USA. Phosphate buffered saline (1 × PBS, pH = 7.2~7.4) and fetal bovine serum (FBS) were purchased from Gibco, Grand Island, NY, USA. The 0.9% sodium chloride for injection (0.9% NaCl) and the 5% glucose for injection (5% Glu) were purchased from Shijiazhuang No. 4 Pharmaceutical, China. Ultrapure water (H₂O) was obtained from a Millipore system (Millipore, Bedford, MA, USA). All other chemicals were of analytical grade.

Ethyl alcohol (Zorka Pharma, Šabac, Serbia, 96%, v/v) and distilled water, as a mixture, were used as extraction agents. Methyl alcohol (HPLC grade), Folin-Ciocalteu reagent, gallic acid, catechin hydrate, aluminum chloride, hydrochloric acid, sodium nitrate, sodium chloride, sodium hydroxide, sodium hydrogen carbonate, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), potassium persulfate, salivary α-amylase solution, pancreatin from porcine pancreas, bile acid mixture from bovine and ovine mucin, mucin, and pepsin from porcine stomach were purchased from Sigma-Aldrich, Darmstadt, Germany. L-3,4-dihydroxyphenylalanine, 5,5′-Dithiobis(2-nitrobenzoic acid), acetylcholinesterase from Electrophorus electricus (electric eel) Type-VI-S (EC 3.1.1.7), butyrylcholinesterase from equine serum (EC 3.1.1.8), acetylthiocholine iodide, butyrylthiocholine chloride, kojic acid, alpha-amylase ex-porcine pancreas (EC 3.2.1.1), acarbose, p-iodonitrotetrazoliumviolet (>95%), α-glucosidase solution from Saccharomyces cerevisiae (EC 3.2.1.20), Lugol reagents (diluted iodine-potassium iodide solution), ABTS, DPPH, tryptic soy broth (TSB), and formic acid were obtained by Sigma Aldrich, St. Louis, MO, USA. Gentamicin (solution for injection, 40 mg/mL) was bought from Panfarma (Belgrade, Serbia).

The non-waterproof graphite felt (cathode) and the 160K graphite fiber brush (anode) were purchased from Beijing Sanye Carbon Co. (China).
The 2,4-DClNB (purity > 99.9 %), 2,4-DClAn (purity > 99.9 %), 4-ClAn (purity > 99.9 %), and 2-ClAn (purity > 99.9%) were all analytical grade and purchased from Sinopharm Chemical Reagent Co. (China). Sucrose, KH₂PO₄, Na₂HPO₄∙3H₂O, NaHCO₃ were all analytical grade and purchased from Sinopharm Chemical Reagent Co., Ltd. (China). Methyl alcohol (HPLC grade) was purchased from Sigma-Aldrich Co. (America).