Ab10401

Manufactured by Abcam

Sourced in United States

Ab10401 is a laboratory product manufactured by Abcam. It is a lab equipment item, but a detailed description cannot be provided while maintaining an unbiased and factual approach without extrapolation or interpretation.

Automatically generated - may contain errors

Lab products found in correlation

Human bm mscs, by American Type Culture Collection (1 mentions) Dmem low glucose, by Fujifilm (1 mentions) Be 2 c, by American Type Culture Collection (1 mentions) Ab18672, by Abcam (1 mentions) Ab10400, by Abcam (1 mentions) Ab188776, by Abcam (1 mentions) Biocoat matrigel invasion chamber, by Corning (1 mentions) Diff quik, by Sysmex (1 mentions)

2 protocols using ab10401

Neuroblastoma and BM-MSCs Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols

The neuroblastoma cell line BE(2)‐C was purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA) and cultured in DMEM and Ham's F‐12 (DMEM/F‐12; Wako, Osaka, Japan) supplemented with 10% FBS. We purchased human BM‐MSCs from the ATCC and maintained them in low‐glucose DMEM (Wako) supplemented with 10% FBS.
Recombinant human CXCL1 and CXCL2 were obtained from R&D Systems (Minneapolis, MN, USA; 275‐GR and 276‐GB). A CXCR2‐specific antibody for neutralization was purchased from Abcam (ab10401; Cambridge, MA, USA).

Hashimoto O., Yoshida M., Koma Y., Yanai T., Hasegawa D., Kosaka Y., Nishimura N, & Yokozaki H. (2016). Collaboration of cancer‐associated fibroblasts and tumour‐associated macrophages for neuroblastoma development. The Journal of Pathology, 240(2), 211-223.

+ Open protocol

+ Expand

Transwell Migration and Invasion Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

For the migration assay, TE cells (5.0 × 10⁵ cells/ well) in serum-free media were plated on the upper transwell inserts with an 8-μm pore filter (BD Falcon, Lincoln Park, NY) in 24-well plates. For the invasion assay, TE cells (5.0 × 10⁵ cells/ well) in serum-free media were plated on the inserts of a Corning^® BioCoat™ Matrigel^® Invasion Chamber (Corning, Tewksbury, MA) in 24-well plates. Medium containing 1% FBS was added in the lower chamber. Recombinant human CXCL8, inhibitors, or neutralizing antibodies were added in the upper chamber and incubated at 37°C. After 24 h or 48 h, the cells were stained with Diff-Quik (Sysmex, Kobe, Japan), and then the cells on the upper surface of the membrane were removed with a cotton swab and air-dried. Five images at 100× magnification were obtained from each membrane with a CCD camera, and the number of cells was counted. The inhibitors against PI3K (LY294002) and MEK1/2 (PD98059) were purchased from Cell SignaIing Technology. The neutralizing antibodies were as follows (all from Abcam): normal mouse IgG (#ab188776); mouse antibody against CXCR1 (#ab10400); mouse antibody against CXCR2 (#ab10401); mouse antibody against CXCL8 (#ab18672).

Hosono M., Koma Y.I., Takase N., Urakawa N., Higashino N., Suemune K., Kodaira H., Nishio M., Shigeoka M., Kakeji Y, & Yokozaki H. (2017). CXCL8 derived from tumor-associated macrophages and esophageal squamous cell carcinomas contributes to tumor progression by promoting migration and invasion of cancer cells. Oncotarget, 8(62), 106071-106088.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!