Control lentivirus

The NSCLC cell line A549 was obtained from Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Non-fetal-derived human brain microvascular endothelial cells (HBMECs) were purchased from Bioleaf Biotechnology (Shanghai, China). A549 and HBMECs were grown in Roswell Park Memorial Institute-1640 medium (Invitrogen, Carlsbad, CA, USA) containing 10% fetal bovine serum at 37 °C with 5% CO₂. The lnc-MMP2-2 overexpression and silencing lentivirus and their control lentivirus were packaged by Genomeditech (Shanghai, China). EPB41L5-targeting siRNA and scramble control siRNA were obtained from Ribobio (Guangzhou, China). The EPB41L5-targeting sequences were as follows: siRNA#1, 5′-GGATCACGATTTA GATATA-3′; siRNA#2, 5′-GTCCTGAACTTGTCTCAGA-3′; siRNA#3: 5′-CGACTATTTTGGTCTGAG A-3′. The overexpression plasmid (pcDNA3.1-EPB41L5) and the empty vector (pcDNA3.1) were obtained from Genomeditech (Shanghai, China). miR-1207-5p antagomir, antagomir control, and miR-1207-5p agomir and agomir control were purchased from Ribobio (Guangzhou, China). Cell transfection was performed using Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA) following the manufacturer’s instructions. For exosome transfection, lnc-MMP2-2 Smart Silencer (RiboBio Co, Ltd., Guangzhou, China) were loaded in exosomes using the Exo-Fect Exosome Transfection Kit.

We silenced three sequences of the CD147 gene, namely, CD147‐Homo‐550 (siRNA), CD147‐Homo‐647 (siRNA) and CD147‐Homo‐702 (siRNA). We checked the efficiency of the three interference sequences and selected the interference of CD147‐Homo‐550 (siRNA), then packaged it as lentivirus to use in this study (Figure S2C). HN4, HN6 and HN30 cells were transfected with shCD147 lentivirus or control lentivirus (Genomeditech, Shanghai, China) according to the manufacturer's instruction. And the indicated cells were treated with 5 μg/mL puromycin (MedChem Express, China) for 2 weeks to establish stable cell lines. The three interference senquence of CD147 (siRNA) was as follow: CD147‐Homo‐550 (siRNA) sence: CCAGAGUGAAGGCUGUGAATT; CD147‐Homo‐550 (siRNA) antisence: UUCACAGCCUUCACUCUGGTT; CD147‐Homo‐647 (siRNA) sence: GGCCUGGUACAAGAUCACUTT; CD147‐Homo‐647 (siRNA) antisence: AGUGAUCUUGUACCAGGCCTT; CD147‐Homo‐702 (siRNA) sence: AGCAGGUUCUUCGUGAGUUTT; CD147‐Homo‐702 (siRNA) antisence: AACUCACGAAGAACCUGCUTT.