Ab137721

LUHMES cells in each group were grown on coverslips, fixed with 4% paraformaldehyde for 20 min, and permeated using 0.5% Triton X-100 (Sigma-Aldrich) for 20 min. For the tissue sections, the slices of substantia nigra and corpus striatum were dewaxed with xylene, treated with gradient alcohol, and washed with PBS. Subsequently, the slices were subjected to antigen repair by microwave with citrate. After cooling to room temperature, the slices were treated with 3.3% H₂O₂ at 37°C for 20 min. After washing and blocking, the samples were incubated with anti-ACSL4 (1 : 50 dilution, Abcam, ab227256) and anti-TH (1 : 50 dilution, Abcam, ab137721) overnight at 4°C, then with the corresponding secondary antibody (Abcam) for 1 hour. The nuclei were stained using 10 g/mL DAPI, and the results were immediately photographed under a fluorescence microscope. For details of other experimental methods, please refer to the supplementary materials (available here).

Primary antibodies against pSer129 α-syn (#015-25191) and IBA-1 (#019-19741) were purchased from Wako Pure Chemical Industries (Richmond, VA, USA). The primary antibody against glial fibrillary acidic protein (GFAP, #RA22101) was purchased from Neuromics (Edina, MN, USA). Primary antibodies against CD3 (#ab16669) and tyrosine hydroxylase (TH, #ab137721) were purchased from Abcam (Cambridge, UK). The primary antibody against F4/80 (#sc-377009) was purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Primary antibody against NCR1 (#PA5-102,860) was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Fexofenadine (#F9427) was purchased from Sigma Aldrich (St. Louis, MO, USA).