All reactions were performed under an atmosphere of nitrogen. DMF and CH2Cl2 were dried using an INERT solvent purification system. Chromatographic purification was performed on silica gel (Macherey-Nagel, 230–400 mesh) with the indicated eluents. Size-exclusion chromatography was carried out on Bio-Beads S-X1 beads (200–400 mesh) with the indicated eluents. All other solvents and reagents were of reagent grade and used as received. Phthalocyanine 3 was prepared as described previously.14 (link)MALDI mass spectra were recorded on a Bruker Autoflex Speed MALDI-TOF mass spectrometer. For accurate mass measurements, the lowest m/z value of the isotopic envelope was reported and compared with the theoretical value. Electronic absorption and steady-state fluorescence spectra were taken on a Shimadzu UV-1800 UV-Vis spectrophotometer and a Horiba FluoroMax spectrofluorometer respectively. HPLC experiments were performed on an Apollo-C18 column (5 μm, 4.6 μm × 250 mm) or on an XBridge-C18 column (5 μm, 10 μm × 250 mm) by using a Waters 1525 binary HPLC pump with a Waters 2998 photodiode array detector.
Uv 1800 uv vis spectrophotometer
Manufactured by Horiba
The UV-1800 UV-Vis spectrophotometer is a compact and versatile instrument for measuring the absorption or transmission of light in the ultraviolet and visible regions of the electromagnetic spectrum. It features a wavelength range of 190 to 1100 nanometers and can be used for a variety of analytical applications.
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Lab products found in correlation
Autoflex speed maldi tof mass spectrometer, by Bruker (1 mentions)
Fluoromax spectrofluorometer, by Horiba (1 mentions)
Quantamaster spectrofluorometer, by Horiba (1 mentions)
Novostar fluorescence plate reader, by BMG Labtech (1 mentions)
Chelex, by Bio-Rad (1 mentions)
Henelaser source, by Malvern Panalytical (1 mentions)
Digital micrograph, by Ametek (1 mentions)
Zetasizer ultra instrument, by Malvern Panalytical (1 mentions)
300 mesh au grid, by Ted Pella (1 mentions)
Dispersion technology software, by Malvern Panalytical (1 mentions)
3 protocols using uv 1800 uv vis spectrophotometer
1
Synthesis and Characterization of Phthalocyanine Dye
2
Spectroscopic Characterization of MFF
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Absorbance and fluorescence spectra of MFF in 10 mM Tris–HCl buffer containing 0.1% SDS, pH 8.0 were obtained at 23 °C using a Shimadzu UV-1800 UV-VIS Spectrophotometer and a Photon Technology International Quanta Master spectrofluorometer (HORIBA Scientific), respectively. Quenching of MFF (4 μM) by FAS and FeCl3 was measured using a NOVOstar fluorescence plate reader (BMG Labtech). Aqueous FAS was prepared with equimolar ascorbic acid to prevent oxidation. Tris buffer was treated with Chelex (1421253, BioRad) overnight to remove trace heavy metals. Fluorescence spectra were uncorrected for lamp intensity and detector sensitivity.
3
Characterization of Quantum Dots and Gold Nanoparticles
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Three different techniques
were used to characterize the QDs and AuNP used in this study: (1)
Electronic absorption and PL emission spectra were recorded using
a Shimadzu UV-1800 UV–vis spectrophotometer and a Horiba, Inc.
fluorometer (excitation at λ = 395 nm), respectively. (2) Dynamic
light scattering (DLS) was used to measure hydrodynamic size. The
samples were transferred into a square-shaped capillary, and measurements
were recorded on a ZetaSizer Ultra instrument equipped with a HeNe
laser source (λ = 633 nm) (Malvern Instruments Ltd., Worcestershire,
UK) and analyzed using Dispersion Technology Software (Malvern Instruments
Ltd.) as previously described.20 (link) (3) Structural
characterization and elemental analysis of the as-prepared NPs were
carried out using a JEOL 2200-FX analytical high-resolution transmission
electron microscope with a 200 kV accelerating voltage. TEM samples
were prepared by spreading a drop (5–10 μL) containing
the NPs onto an ultrathin carbon/holey support film on a 300 mesh
Au grid (Ted Pella, Inc.) and letting it dry. The concentration of
NPs used for TEM was 50–100 nM. Individual particle sizes were
measured using a Gatan digital micrograph (Pleasanton, CA, USA); average
sizes along with standard deviations were extracted from analysis
of at least 50–100 nanoparticles.
were used to characterize the QDs and AuNP used in this study: (1)
Electronic absorption and PL emission spectra were recorded using
a Shimadzu UV-1800 UV–vis spectrophotometer and a Horiba, Inc.
fluorometer (excitation at λ = 395 nm), respectively. (2) Dynamic
light scattering (DLS) was used to measure hydrodynamic size. The
samples were transferred into a square-shaped capillary, and measurements
were recorded on a ZetaSizer Ultra instrument equipped with a HeNe
laser source (λ = 633 nm) (Malvern Instruments Ltd., Worcestershire,
UK) and analyzed using Dispersion Technology Software (Malvern Instruments
Ltd.) as previously described.20 (link) (3) Structural
characterization and elemental analysis of the as-prepared NPs were
carried out using a JEOL 2200-FX analytical high-resolution transmission
electron microscope with a 200 kV accelerating voltage. TEM samples
were prepared by spreading a drop (5–10 μL) containing
the NPs onto an ultrathin carbon/holey support film on a 300 mesh
Au grid (Ted Pella, Inc.) and letting it dry. The concentration of
NPs used for TEM was 50–100 nM. Individual particle sizes were
measured using a Gatan digital micrograph (Pleasanton, CA, USA); average
sizes along with standard deviations were extracted from analysis
of at least 50–100 nanoparticles.
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