Fluorpen 100

Manufactured by Photon Systems Instruments

Sourced in Czechia

The FluorPen 100 is a portable, hand-held device designed for measuring the fluorescence parameters of plants and other photosynthetic organisms. It is capable of non-invasive assessment of various photosynthetic properties, including the quantum yield of Photosystem II.

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Lab products found in correlation

Spad meter, by Konica Minolta (1 mentions)

3 protocols using fluorpen 100

Leaf Physiology and Root Imaging

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Prior to removal from soil, plant vessels were opened and leaves were dark-adapted for 25 min; physiological measures were then collected with a FluorPen 100 instrument (Photon Systems Instruments, Drasov, Czech Republic) according to manufacturer's instructions. Plants were then removed from soil matrix, washed briefly in sterile water to remove soil and then roots were spread for imaging. Root area was measured by thresholding image then counting white pixels using ImageJ (Schneider et al., 2012 (link)). Plant tissues were frozen in liquid nitrogen and stored at −80°C for molecular analysis.

Timm C.M., Pelletier D.A., Jawdy S.S., Gunter L.E., Henning J.A., Engle N., Aufrecht J., Gee E., Nookaew I., Yang Z., Lu T.Y., Tschaplinski T.J., Doktycz M.J., Tuskan G.A, & Weston D.J. (2016). Two Poplar-Associated Bacterial Isolates Induce Additive Favorable Responses in a Constructed Plant-Microbiome System. Frontiers in Plant Science, 7, 497.

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Plant Growth and Photosynthetic Efficiency

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The longest root and shoot were measured by a digital caliper. The dry weight of root and shoot was taken after drying for 3d at 80 °C in an electric oven. The chlorophyll score was measured on young leaves by SPAD meter (Minolta, Japan). Chlorophyll a fluorescence kinetics (OJIP) such as Fv / Fm (photosystem II quantitative efficiency) and Pi ABS (photosynthesis index), were also measured by FluorPen 100 (photon systems instruments, Czech Republic) in dark-adapted young leaves for 1 h.

Prity S.A., El-Shehawi A.M., Elseehy M.M., Tahura S, & Kabir A.H. (2021). Early-stage iron deficiency alters physiological processes and iron transporter expression, along with photosynthetic and oxidative damage to sorghum. Saudi Journal of Biological Sciences, 28(8), 4770-4777.

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Protonemata Photosynthetic Response to Osmotic Stress

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The maximal rate of PSII was determined by variable fluorescence (Fv)/maximal fluorescence (Fm) measurements. Briefly, 10-day old protonemata were exposed to 0 and 300 mM of either glucose or sorbitol for 24 h. Dark adaptation for 10 to 15 min was allowed before each measurement. Then Fv/Fm was measured using the fluorimeter FluorPen100 (Photon Systems Instruments, Czech Republic).

Chamorro-Flores A., Tiessen-Favier A., Gregorio-Jorge J., Villalobos-López M.A., Guevara-García Á.A., López-Meyer M, & Arroyo-Becerra A. (2020). High levels of glucose alter Physcomitrella patens metabolism and trigger a differential proteomic response. PLoS ONE, 15(12), e0242919.

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