Prepared targets were analyzed using an microflex LT/SH MALDI-TOF mass spectrometer (Bruker Daltonics GmbH, Bremen, Germany) equipped with the nitrogen UV laser in positive mode. Spectra were collected manually using the manufacturer's flexControl version 3.4 software, using the parameters described in a previous paper 13 (link) . Collected spectra were subjected to Savitzky-Golay smoothing, baseline correction with the TopHat algorithm and calibration using BTS in quadratic mode using the manufacturer's flexAnalysis version 3.4 software. Each sample was measured at least five times. Collected spectra were used for microorganisms identification using the MALDI Biotyper 4.1 platform (Bruker Daltonics GmbH, Germany) according to Bruker guidelines, as described by Maslak et al. 14 (link) .
Microflex lt sh maldi tof mass spectrometer
Manufactured by Bruker
Sourced in Germany
The Microflex LT/SH MALDI-TOF mass spectrometer is a laboratory instrument designed for the analysis of a wide range of samples using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. The core function of this equipment is to ionize and analyze samples, providing detailed information about their molecular composition.
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Lab products found in correlation
2 protocols using microflex lt sh maldi tof mass spectrometer
1
MALDI-TOF Mass Spectrometry for Microbial Identification
2
Antimicrobial Susceptibility of Pseudomonas aeruginosa
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The isolated JNQH-PA57 was grown in Mueller-Hinton agar (MHA) (Oxoid, Hampshire, United Kingdom) at 37 °C for 24 h. The species identification was determined with Microflex LT/SH MALDI-TOF mass spectrometer (Bruker, Germany). The antimicrobial susceptibility of this stain was performed using MIC evaluation via the E-test method for the following antimicrobial agents: piperacillin, piperacillin/tazobactam, ticarcillin/clavulanic acid, ceftazidime, cefepime, aztreonam, imipenem, meropenem, amikacin, tobramycin, levofloxacin, ciprofloxacin, according to the manufacturer’s guidelines (AB Biodisk, Sweden). For colistin, the MIC was determined via a broth microdilution method, according to the Clinical and Laboratory Standards Institute (CLSI) guideline. P. aeruginosa ATCC 27853 served as a quality control strain for susceptibility testing. The interpretation of the results was based on the CLSI 2020 guideline [55 ].
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