Log In Sign up
The largest database of trusted experimental protocols

Plan apo λ 60x oil objective

Manufactured by Nikon
Visit Supplier

The Plan Apo λ 60X Oil objective is a high-quality microscope objective lens designed for use in laboratory and research applications. It features a numerical aperture of 1.42 and a working distance of 0.13 mm, providing excellent optical performance and resolution. The objective is plan-apochromatic, ensuring flat field imaging and minimized chromatic aberrations. It is designed for use with oil immersion techniques.

Automatically generated - may contain errors

Lab products found in correlation

A1r laser scanning confocal microscope, by Nikon (1 mentions) Orca flash 4.0 c11440, by Hamamatsu Photonics (1 mentions) Tetraspeck microsphere, by Thermo Fisher Scientific (1 mentions) Eclipse ti2 microscope, by Nikon (1 mentions)

2 protocols using plan apo λ 60x oil objective

1

Cheese Microstructure Analysis during Ripening

Check if the same lab product or an alternative is used in the 5 most similar protocols
The microstructure of cheese samples, ripened for 0, 30 and 60 days, was analysed using a Nikon A1r laser confocal scanning microscope, equipped with a Plan Apo λ 60X Oil objective, detector Galvano, with a pinhole size of 69 μm and a pixel size of 0.04 µm. The samples were cut using a blade and the proteins were stained with 0.2% Fast Green, while fats were stained with 0.5% Nile Red diluted in distilled water. We used an average of 2 modes in channels series, as follows: channel 1: Nile Red: λexc = 488 nm, λem = 563 nm; channel 2: Fast Green: λexc = 633 nm, λem = 650 nm.
Florio M., Cimini C., Ianni A., Bennato F., Grotta L., Valbonetti L, & Martino G. (2023). New Insight into the Quality Traits of Milk and Cheese from Teramana Goats, a Native Italian Breed. Animals : an Open Access Journal from MDPI, 13(8), 1344.
+ Open protocol
+ Expand
2

Epifluorescence Microscopy for Multi-Channel Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols
Epifluorescence microscopy was performed on Nikon Eclipse Ti2 microscope with Nikon Plan Apo λ 60x Oil objective and Intermediate Magnification switching of 1.0x/1.5x. The system was driven by NIS-Elements AR software. Illumination was provided by high power LED. Emission was collected using filter sets: ET - Sedat Quad (Chroma, 89100) for Hoechst 33342 channel, ET - Gold FISH (Chroma, 49304) for Cy3 or ATTO550 channel, ET - Cy5 Narrow Excitation (Chroma, 49009) for Cy5 channel, and ET - Cy7 (Chroma, 49007) for Alexa750 channel. Images were recorded as z-stacks (21 to 35 steps), with 200 nm or 300 nm step size using a digital CMOS camera (ORCA-Flash 4.0 C11440, Hamamatsu), except for several images which were recorded using an EMCCD camera (Andor iXon 888) (Figures 3F, S3D and S3E). TetraSpeck™ Microspheres (T7279, Invitrogen) were also imaged in the same way for correction of chromatic aberration between Cy3/ATTO550 channel and Cy5 channel.
Wang Y., Cottle W.T., Wang H., Feng X.A., Mallon J., Gavrilov M., Bailey S, & Ha T. (2021). Genome Oligopaint via Local Denaturation Fluorescence in Situ Hybridization (GOLD FISH). Molecular cell, 81(7), 1566-1577.e8.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!