Analysis of fluorescent reporter expression and gonad phenotypes were performed under Nomarski optics on a Zeiss Axioplan2 microscope with a Zeiss AxioCam MRm CCD camera. Plate phenotypes were observed using a Leica MZ16F dissecting microscope with a Hamamatsu C4742-95 CCD camera.
C4742 95 ccd
Manufactured by Hamamatsu Photonics
The C4742-95 CCD is a charge-coupled device (CCD) image sensor manufactured by Hamamatsu Photonics. It is designed to capture and convert light signals into electrical signals, enabling the detection and measurement of various types of radiation, including visible light, ultraviolet, and near-infrared. The core function of the C4742-95 CCD is to provide high-sensitivity, low-noise image capture capabilities for a wide range of scientific and industrial applications.
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2 protocols using c4742 95 ccd
1
Fluorescent Microscopy of Gonads
2
Fluorescent Imaging of Immobilized Animals
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Images were acquired using HCImage software (Hamamatsu) and an Olympus SZX2 stereo microscope attached to a Lumen Dynamics X-Cite Fluorescence Illumination System for fluorescent detection assays. Animals were immobilized by chilling on NG agar plates to 4° prior to photographing. After initial image capture, subsequent analysis of images was performed using ImageJ software (NIH). For display of the injected animals, two consecutive images were overlaid using ImageJ. The scale bar was measured using a hemocytometer. For Figure 6 , bright field and fluorescent images were acquired using Openlab 3.1.7 (Improvision), a C4742-95 CCD (Hamamatsu), and a Zeiss Axiophot.
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