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Bovine serum fraction 5

Manufactured by Merck Group
Sourced in Germany

Bovine serum fraction V is a laboratory-grade product derived from bovine serum. It is a purified and concentrated protein fraction that is commonly used in cell culture media and other biological applications. The primary function of Bovine serum fraction V is to provide a source of proteins, growth factors, and other essential components required for the growth and maintenance of cell cultures.

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2 protocols using bovine serum fraction 5

1

Quantification of Flavonoid Standards

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The standards of flavonoids were purchased from Extrasynthese (Genay, France) except for kaempferid (Fluka, Buchs, Germany). HPLC-grade solvents and analytical-grade chemicals NaCl, KCl, NaHCO3, CaCl2.2H2O, FeSO4, D-glucose, 2,2’-dinitro-5,5’-dithiodibenzoic acid (DTNB) and CCl4 were provided by Merck (Darmstadt, Germany). HEPES, EGTA, albumin, bovine serum fraction V, minimum 98%, 2-thiobarbituric acid (4,6-dihydroxypyrimidine-2-thiol; TBA), and t-BuOOH were provided by Sigma-Aldrich (Germany). In our experiments, pentobarbital sodium (Sanofi, France), KH2PO4 (Scharlau Chemie SA, Spain), MgSO4.7H2O (Fluka AG, Germany), trichloroacetic acid (TCA), ascorbinic acid and glycerol (Valerus, Bulgaria), and lactate dehydrogenase (LDH) kit (Randox, UK) were used.
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2

Biochemical Analysis of Tissue Samples

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The following chemicals and reagents, including pentobarbital sodium (Sanofi, France), HEPES (Sigma Aldrich, Germany), NaCl (Merck, Germany), KCl (Merck), D-glucose (Merck), NaHCO3 (Merck), KH2PO4 (ScharlauChemie SA, Spain), K2HPO4 (ScharlauChemie SA, Spain), glycerol (Scharlau-Chemie SA, Spain), CaCl2x2H2O (Merck), MgSO4x 7H2O (Fluka AG, Germany), collagenase from Clostridium histolyticum type IV (Sigma Aldrich), albumin, bovine serum fraction V, minimum 98% (Sigma Aldrich), EGTA (Sigma Aldrich), 2-thiobarbituric acid (4,6-dihydroxypyrimidine-2-thiol; TBA) (Sigma Aldrich), trichloroacetic acid (TCA) (Valerus, Bulgaria), 2,2'-dinitro-5,5'-dithiodibenzoicacid (DTNB) (Merck), lactate dehydrogenase (LDH) kit (Randox, UK) were used.
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