Phospho camkii thr286 rabbit mab

Cells or tissues were lysed using RIPA buffer (Cell Signaling Technology, 9806). Protease Inhibitor Cocktail (Cell Signaling Technology, 5871) was added to the lysates. Proteins were separated by SDS–PAGE and transferred to nitrocellulose membranes to assess abundance by primary antibody detection. The primary antibodies used were Phospho‐CaMKII (Thr286) Rabbit mAb (1:1,000; Cell Signaling Technology, cat# 12716), CaMKII (pan) Rabbit mAb (1:1,000; Cell Signaling Technology, cat# 4436), Phospho‐Elk‐1 (Ser383) Antibody (1:1,000; Cell Signaling Technology, 9181), Elk‐1 Antibody (1:1000; Cell Signaling Technology, cat# 9182), p38 MAPK Antibody (1:1,000; Cell Signaling Technology, cat# 9212), Phospho‐p38 MAPK (Thr180/Tyr182) Antibody (1:1,000; Cell Signaling Technology, cat# 9211), Anti‐c‐Fos (phosphor T232) antibody (1 μg/ml; Abcam, cat# ab43175) and Anti‐c‐Fos (phosphor T232) antibody (1 μg/ml; Abcam, cat# ab90289), Anti‐GRO alpha antibody (4 μg/ml Abcam, cat# ab86436), and Monoclonal Anti‐β‐Actin (1 μg/ml; Sigma, cat# A1978). Signals were visualized using ECL Western Blotting Substrate (Thermo Fisher Scientific, 32106). Band intensities were quantified using the ImageJ software for grayscale statistics.